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BACTERIAL CONCRETE

Muhammed P H
Jeni Joy
GUIDED BY: Jiya George
Asst. Prof. Veena S Kumar Annmary C J
 INTRODUCTION 2

 Micro cracks are the main cause to structural failure.

 As a result water and other chemicals enters in to the

structure.

 It cause corrosion of steel reinforcement.

 One way to repair these cracks is by an automatic

mechanism.

 It is done with the help of calcite precipitating bacteria.

 It precipitate calcite mineral and fill the cracks in the

concrete.
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OBJECTIVES
 To study the effects of adding bacteria to concrete.

 To study the strength charatristics in concrete due

to bacterial action.
 To observe the change in density due to the addition
of bacteria.
 CRITICAL REVIEW
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The journal we selected is “ A STUDY ON STRENGTH

CHARACTERISTICS OF BACTERIAL CONCRETE”
[Dept. of civil engineering, Mar Athanasius college
,Kothamangalam.]
MATERIALS REQUIRED:
 Cement- OPC of grade 53.
 Coarse aggregate
 Fine aggregate
 Water
 BASCILLUS SUBTILIS of strain JC3
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 Bacillus Subtilis JC3 bacteria of concentration 108
cells/ml was used.

 Bacteria is diluted at concentration of 103, 105, & 107

cells/ml.

 M30 grade concrete is used.

 Bacteria added at different concentrations.

 The bacteria is collected from the microbiology dept. of

horticulture college, Mannuthy.
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 These are then mixed together with the concrete

mixture at different percentage .

 The specimens of M30 grade are made with and without

bacteria.

 Compressive strength test, Splitting tensile strength

tests ,flexural strength test are to be done.

 Then the results are compared.

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METHODOLOGY
 A specific group of alkali resistant spore forming
bacteria of genus Bacillus are selected.

 The bacteria we use here is Bacillus Subtilis.

 These are added to the concrete mixture.

 Standard cubes ,cylinders and beams are made.

 Compressive strength, tensile strength etc are done.

 Compare the result with conventional concrete.

ABOUT BACILLUS SUBTILIS 8

 It is an aerobic, spore forming bacteria.

 It also lives in anerobic condition.

 It will become active in the presence of oxygen.

 In unfavourable condition, it form spores and in

favourable condition it will break the spore and get
active.

 Due to it's spore forming ability, it can live about 300

years.

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Microscopic view of bacillus subtilis

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WORKING PROCESS

 Bacteria is supplied with calcium-based nutrient.

 When cracks appears in structure, water and air enters
into the structure.
 Bacteria get active when in contact with moisture and
air.
 Then the calcium source in the bacteria forms calcium
hydroxide

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Reaction involved 11

 Ca(OH)2 + CO2 CaCO3 + H₂O

 Ca(OH)2 acts as catalyst.

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WORK DONE 12

 Literature review
 Mix proportion
 Material collection
 Testing of materials
 Culturing of bacteria
 Casting
 Testing of specimens
 Analysis of result
Mix proportion 13

According to IS10262 ,for 1 m3 M30 concrete:

 cement - 450 kg/m3

 water -197kg/m3

 Fine aggregate – 797kg/m3

 Coarse aggregate – 1048kg/m3

 water cement ratio – 0.42

 RESULT AND DISCUSSION 14
• Testing of materials
Coarse aggregate Fine aggregate

Specific gravity 2.83 2.74

Bulk density 1.6 kg/l 1.7 kg/l

Sieve analysis 2.76 4.03

• Culturing of bacteria 15

 The bacteria ‘BACILLUS SUBTILIS JC3’ was collected from

Agricultural center, Mannuthy.

 Nutrient broth were prepared for the growth of bacterias by

the help of biotechnology department of our college.

 Several processes like sterilization and inoculation of bacteria

was done.

 It is then kept in a shaker for the growth according to the

concentration required.

 Concentration was measured with the help of uv visible

spectrophoto meter.
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• Casting
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CONCRETE TYPE CASTED DATE

CONVENTIONAL CONCRETE 02/03/2019

103 cells/ml 20/03/2019

105 cells/ml 16/03/2019

107 cells/ml 22/03/2019

109cells/ml 04/05/2019
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• Testing of specimens
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Compressive strength of M30 grade concrete

Concrete type 7th day 28thday
strength(N/mm2) Strength (N/mm2)

Conventional 21.06 29.21

concrete

103 cells/ml 22.04 38.51

105 cells/ml 25.98 39.67

107 cells/ml 27.46 41.41

109 cells/ml 25.74

Graph of 7th day compressive
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strength

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Graph of 28thday compressive
strength 22

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Spliting tensile strength test

Concrete type 28th day strength (N/mm2)

Conventional concrete 1.834

103 cells/ml 2.333

105 cells/ml 2.614

107 cells/ml 2.913

109 cells/ml
Graph of 28thday spliting tensile
strength 25

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Flexural strength test 27

Concrete type 28th day strength (N/mm2)

Conventional concrete 5.41

103 cells/ml 6.41

105 cells/ml 7.25

107 cells/ml 8.33

109 cells/ml
Graph of 28thday flexural strength 28

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Density of M 30 grade concrete

Concrete type Density (Kg/m3)

Conventional concrete 2300

103 cells/ml 2400

105 cells/ml 2490

107 cells/ml 2520

109 cells/ml
Analysis of results 31

percentage increase in 28th day strength

Concrete type Increase in Increase in Increase in

compressive spliting tensile flexural
strength (%) strength (%) strength(%)

BC,103 cells/ml 31.83 27.32 18.48

BC, 105 cells/ml 35.80 42.62 34.01

BC,107 cells/ml 41.76 59.01 53.97

BC,109 cells/ml
Conclusion
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 From the experiments conducted, we infer that bacterial

concrete can effectively increase the strength of concrete by
closing the micropores in concrete and making it denser.

 The 7th and 28th day strength of all specimens of bacterial

concrete are stronger than conventional concrete.

 The optimum concentration of cells are found to be 107

cells/ml from the experimental results.

 Percentage increase in strength is greater in splitting tensile

strength than other two.
 References 33
 Jacob V. James (Department of civil engineering, Muthoot Institute of
Science & Technology)April 2017

 Savitha Jose, Reshma George, Alina Rodrigues, Jisbin John, Devika

Venu (Dept. of Civil Engg, Mar Athanasius Collegeof Engineering) March
2018

 L. Soundari, C.S.Maneesh Kumar, S.Anthoniraj, E.Karthikeyan

(Department of civil Engg, IFET college of Engineering) December 2015

 Phani Kumar.V, V.Sainath, B.Ashok Varma (Asst.Professors,

Gudlavalleru Engineering College) March 2017

 Neha singla, Sanjay K Sharma, Jasvir singh Rattan(Civil Engg

Department, NITTTR Chandigarh)