Beruflich Dokumente
Kultur Dokumente
Recombinant DNA
Technology and Genomics
Agarose Gel Electrophoresis
Size
and
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Agarose Gel Electrophoresis
Electrophoresis Animation
Recombinant DNA
Griffith’s experiment
Transformation
Bacterial Transformation
Bacteria, such as E.coli, can take up and express
foreign DNA, usually in the form of a plasmid.
Transformation
deoxynucleotide dideoxynucleotide
Sequencing
Sequencing Animation
Human Genome Project
• Arabidopsis thaliana
• Saccharomyces cerevisiae
• Drosophila melangaster
• Caenorhabditis elegans
• mus musculus
PCR
PCR Animation
PCR
PCR animation
PCR
Cloning by PCR
• Design primer specific for gene of interest (must
know some of the sequence)
• Can use a T-vector because Taq polymerase adds an
A to the 3’ end of sequence
Applications of Recombinant DNA Technology
Chromosomal Location and Gene
Copy Number
Gene microarray
Microarray animation