The Role of VEGF +1612 G>A Polymorphism

on VEGF Serum Levels in Gastritis Patients

dr.Ananda Rahmat Putra

Department of internal medicine,faculty of medicine,university sumatera
utara
INTRODUCTION

 Gastritis is a health problem most often found in clinical practice

 Gastritis is an inflammatory process in the gastric mucosa and submucosa in
response to injury which can be acute or chronic.
 Chronic inflamation is one of the triggers of carcinogenesis
 VEGF is a polypeptide that has spesific mitogenic activity for endothelial
cells.In tumor cells,over – expressions of VEGF can trigger angiogenesis.A well
known tumor supressor protein,p53 has anti-angiogenic effect through VEGF
down regulation pathway
 A Previous study showed that VEGF +1612G>A gene polymorphism was
associated with gastric cancer risk in the Asian population
METHOD

 This study is a cross-sectional design

 Sample was taken from patients with gastritis who came to the Endoscopy
Unit at Haji Adam Malik General Hospital, and Permata Bunda General
Hospital, Medan, Indonesia between April and June 2018.
 Inclusion criteria include gastritis patients diagnosed based on
histopathological examination, at least 18 years old, and willing to take part
in the study
 Exclusion criteria are as follows: patients with systemic disease and
malignancy
 Data analysis was performed through univariate, bivariate (Kruskal Wallis and
Mann Whitney U tests) analyses using SPSS 22nd version (SPSS Inc., Chicago).
 A value of p <0.05 was considered statistically significant.
 RESULT
Table 1: Characteristics of Research Table 2. Polymorphism Frequence Distribution
of VEGF +1612G>A In Gastritis Patients

Variabel n = 60
Variable n (%)
Sex
Male 34 (56.7%) a VEGF +1612 G>A
Female 26 (43.3%)
Polymorphism
Age, years 51.5 (19-67) b AA 7 (11.7)
BMI, kg/m2 22.9 + 6.7 c GA 20 (33.3)
Ethnic
GG 33 (55)
Batak 38 (63.4%) a
Javanese 14 (23.3%)
Acehnese 8 (13.3%)

n = total number of subjects

aPercentage
bMedian (min-max)
cMean ± SD
 RESULT
Table 3. VEGF Level in Gastritis Patients Table 4. Difference in VEGF Serum levels among AA,
GA, GG genotypes, and also between A and G alleles
of VEGF +1612 G> A polymorphism
Variable n = 60 VEGF level (pg/mL)
VEGF +1612
median (min – max) P
G>A

Genotype AA 632.4 (396.8 – 2,185.2)# 0.019*

VEGF, median (min- 415 (65.3-
Genotype GA 481.45 (134.5 – 1,385.8)
max), pg/mL 2185.2) Genotype GG 362.6 (65.3 – 1,602.2)

Allele A 523.65 (134.5 – 2,185.2) 0.002*

Allele G 382 (65.3 – 1,602.2)

*p<0.05
# significantly higher than the GG genotype
Discussion

Previous study stated that

In this study, the GG GG genotype (73,2%) was
genotype (55%) of VEGF most commonly found in
Polymorphism +1612 G> A gastritis patients,
was most commonly however, the response of
found. There was a serum AA level of VEGF
significant relationship +1612 G/A polymoprhism
between AA genotype and to the serum VEGF level
A allele of VEGF +1612 was known to be higher
G>A polymorphism with than GA or GG serum
VEGF level in gastritis levels and the results
patients (p<0.05). were known to be
insignificantly associated
(p = 0.333)
CONCLUSION

There was association between VEGF +1612 G>A polymorphism and serum
level of VEGF in gastritis patients.