BC-5000&BC-5150 - Service Training Material - V1.0 - EN (REV)

BC-5000/BC5150 service training
© 2013 Mindray Confidential

Content
 Clinical
 Overview
 Principal
 Installation
 Software
 Hardware
 Liquid system
 Mechanical structure
2 © 2013 Mindray Confidential

clinical—Blood Cells
 Blood consists of 40-50% blood cell and 50-60% plasma.

 Three main parts of blood cell:
red blood cell, white blood cell and platelet.

Five groups of leukocyte
WBC 5-part proportion
Lymphocyte 25 ~ 30%
Monocyte 3 ～8 %
Neutrophil 50 ~70%
Basophil 0 ～1 %
Eosinophil 0.5 ～3%

 Morphology：size, shape, cytomembrane, cytoplasma, nucleus,

granule.
RBC WBC PLT

GRAN MONO LYMPH
NEU ESO BASO

Clinical—Blood Cells

Morphological

Clinical—WBC five part comparison
NEU EOS BASO LYMPH MONO

Diameter 10~14um 11~16um 10~12um 6~9um 12~20um
Cytoplasm Large more more less More than

amount LYM
karyon Multlobed, 2lobes 3~4lobes, Large round Irregular
2~5 mostly or hard and
to indented
divide
Granule Neutral Eosinophilic basophilic None azurophilic
or normally
acidophi
lic
Chromatin More lump More lump More lump Tightness loosen
evenly
Color of Reddish Reddish Reddish Baby blue Grey blue
cytoplas or
8
m
little
reddish
Clinical —granules in cytoplasm
Neutral Eosinophilic basophilic Azurophilic
Size Fine and even Thick and even Most thick and Thicker than
uneven NEU, uneven
Shape Fine Round or ellipse Differ in Differ in

particles shape shape
Amount Much Much Less A few or

moderate
Color Pale violet Orange red or Royal purple Purple red

red or dark yellow or dark purple
reddish
Distributio Even Even Uneven, coat Uneven, coat
n the nucleus the nucleus
normally occasionally

Conten t
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Liquid system

Overview—appearance
BC-5150 BC-5000

Overview —Dim ension
BC-5000
vs BC-2800
324mm 322mm
437mm
415mm
410mm 386mm

Overview —Report parameter
White Blood Cell count WBC Red Blood Cell count RBC
Basophils number Bas# Hemoglobin Concentration HGB
Basophils percentage Bas% Mean Corpuscular Volume MCV
Neutrophils number Neu# Mean Corpuscular Hemoglobin MCH
Neutrophils percentage Neu%
WBC
RBC Mean Corpuscular Hemoglobin Concentration MCHC
11 Eosinophils number Eos#
8
Para
Eosinophils percentage Eos% Para
Red Blood Cell Distribution Width Coefficient RDW-CV
Lymphocytes number Lym# of Variation
Lymphocytes percentage Lym% Red Blood Cell Distribution Width Standard RDW-SD
Monocytes number Mon# Deviation
Monocytes percentage Mon% Hematocrit HCT
Platelet count PLT
Mean Platelet Volume MPV

PLT Platelet Distribution Width PDW BC5150 only
6
Para Plateletcrit PCT
Platelet-large cell ratio P-LCR
Platelet-large cell count P-LCC

Overview —RUO
BC5150 only
Name Abbr.
Abnormal lymphocytes number ALY#
Abnormal lymphocytes Percentage ALY%
Large immature cells number LIC#
Large immature cells percentage LIC%

Overview —appearance
1、Display screen
2、Power/status indicator
3、Probe wipe block
4、Sample probe
5、[ Aspirate] key
 Front of the analyzer

Overview —appearance
1、M-52D diluent inlet

2、Waste outlet
3、Waste sensor
4、AC input socket
5、Power switch
6、Air outlet for fan
7、Back panel
 Back of the analyzer

Overview —Main screen

Overview —difference between BC5000andBC5150
item BC-5000 BC-5150

Test speed 40Samples/h 60Samples/h
Report Para 23 25
RUO NONE 4Para
Sample storage 20000份 40000份
WBC linear 0.00×109/L～100.00×109/L 0.00×109/L～500.00×109/L
PLT linear 0×109/L～1000×109/L 0×109/L ~ 5000×109/L
Front panel 043-002705-00 043-002706-00
Screen assembly 115-018417-00 115-018418-00
Key board 043-002703-00 801-3100-00194-00

Overview —Specification
 Measuring mode：CBC、CBC+DIFF
 Sample mode：WB Capillary W PD
 Sampling：
 WB Capillary W
 Sample volume of aspiration：CBC+DIFF—15ul
CBC—11.5ul
 OV-PD
 Sample volume of aspiration ：200ul
 Diluent dispensed：480ul
 Blood collected：20ul
 Scattergram：DIFF(Report:L-M)，（RUO：L-W、M-W)
 Histogram：WBC、RBC、PLT

Overview —Specification
 Operating temperature：10℃—30℃
 Screen ：10.4’LED，with touch screen
 Weight ：≤25Kg
 Interface ：4USB, 1 ethemet network port
 Data transmission：HL7 supported
 Printer ：external，PCL6
 printer ：EPSON LQ-590K、HP Laser Jet P1505n、HP OfficeJet Pro
K5300、HP LaserJet P1606dn

Overview —reagent
Reagent Name Type
Diluent M-52D DILUENT M-52D
M-52DIFF LYSE M-52DIFF

Lyse
M-52LH LYSE M-52LH
Probe cleanser PROBE CLEANSER /
Control : Mindray m ade

Calibrator : R&D: S-CAL Mindray m ade：S50

目录
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Liquid system

Prin cip le —m ain technology
colorimetry
Laser Lambert
scattering –Beer
law
Impedance
Lyse
Flow
without
cytometry
cyanide
Cytochemical
staining

Principle—system block diagram

Principle—Flow cytometry
 Flow cytometry + Laser scattering—WBC 5DIFF
 Sheath fluid surrounds and forces blood cells suspended in dilution
to pass through the center of flow cell one by one.
Blood cells pass through the facula of flow cell with high speed
cell
Laser facula
Flow cell
sheath
sample

Principle—twice accelerating sheath flow
Scatter
Flow cell
light
Laser
sam ple
2ed Sheath flow 1st sheath flow
Blood sam ple

Principle—Multi-angle laser scattering
 Multi-angle laser scattering；

 LAS—volume of the cells；
 MAS—inner complexity
 WAS—granularity of the cells

Principle—cytochemical staining
 M-52 DIFF Lyse：
 Cationic surfactant To dissolve the RBC，special treatment to WBC
 anionic group stain the granular of the WBC
 Lymph Mono Nue Eos Baso
original
After adding
reagent
More difference in complexity

Principle—cytochemical staining
 M52-LH Lyse
 To beak down the RBC
 All the cells are naked (no membrane) except Baso, the Basophils are
relatively stable in size and inner structure, larger in volume than
other kind of cells,
 To converts hemoglobin to a hemoglobin complex
original
After adding
reagent
Lymph Mono Nue Eos Baso

Principle—scattergram
Neu
Mon
Lym
Baso
Eos

Principle—L-M Scattergram（report）
 DIFF：laser scattering+cytochemical staining
Monocyte
Neutrophil
Lymphocyte
Eosinophil

Principle—histogram of Baso
 laser scattering + cytochemical staining scattergram

histogram
Basophil

Principle—position of cells
Scattergram
DIFF
Histogram
LAS
LIC
LIC
MON NEU
ALY EOS
LYM
Nrbc
BAS
GHO
MAS

Principle—HGB
 HGB measurement
 Lambert-beer law
 HGB(g/L) = Constant×Log 10 (Blank Photocurrent/Sample Photocurrent)
Fe 2+ 3+
Fe
＋ Fe 2+ Fe
3+
Fe 2+ Fe
3+
Fe 2+ Fe
3+
Anionic surfactant
Lyse without cyanide

Special surfactant
ferrous iron is oxidized to ferric iron
Principle—RBC/PLT
 Impedance method

Content
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Liquid system

Installation—procedure
 Preparation
 Installation：check up, unpacking, emplacement, connection,
 Starting up and login
 Setting
 performance verification
 Background, reproducibility
 Quality control and calibration(if needed)
 Scattergram confirmation（blood sample）
 Other setting：customized setting, maintenance, print.
 Customer training

Installation—preparation
 Tools：
 Blade or clipper
 USB flash disk
 Pipette (200ul)
 Cross-headed screwdriver
 Control (normal)
 Calibrator

 Environment：
 should be as free as possible from dust, mechanical vibrations, and
electrical interference;
 Far from brush-type motors, flickering fluorescent lights, and electrical
contacts that regularly open and close;
 Do not place the analyzer on a slope;
 Do not place the analyzer in direct sunlight or in front of a source of heat
or drafts;
 The environment shall be well ventilated

 Space ：shown as in figure

enough room on or below the countertop to accommodate the
diluent and waste containers.
 Voltage： 100V-240V～， 50Hz/60Hz
 Input power： ≤300VA
 Standard configuration： 20cm
 Analyzer, reagents
 Optional：printer , barcode scanner
50cm

Installation-unpackin g
 Check：
 outer packing and apparatus
 Packing list and contents
 Apparatus arrangement

Installation—connection
Power switch
M-52D
diluent inlet
Waste outlet Waste sensor connector

Power socket


M-52DIFF lyse connector

M-52LH lyse connector

Peripheral devices ：
Printer
Barcode scanner
 Computer
Network cable
Data management software
USB port and network port

Installation—Starting up
Hardware self test, liquid system self test and maintenance, background test。

User ID：service
Password：Se s700 （capital S, blank between Se and s700)


Installation—Setting up
reagent
press
press

reagent
press
Scan
or
input

Other settings

Installation—performance test
 Background Verification
Parameter Background/ blank count

requirements
WBC ≤ 0.20 109 / L
RBC ≤ 0.02 1012/ L
HGB ≤1 g/L
HC T ≤0.5 %
PL
T ≤ 10  109 / L



 Reproducibility

 Reproducibility

Installation—quality control
press
Installation—quality control
 Click “By SD” to display the limits in the form of absolute value;
 Or click “By CV” to display the limits in the form of percentage
Content
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Liquid system

Software—calibration
Factory
calibration
factor and
tansfer
factor
with the engineer permission, factory’s Cal factor and transfer factor will
be modified, meanwhile the user’s factor will be adjusted to 100%

Software—calibration
User ’s
factor
only
with the administrator permission, user’s Cal factor is modified, it’s

recommend to be with “Admin” permission to login and calibrate

Software—gain
Target :4.5V
HGB gain can be modified manually, to modified the [set

value] to change the HGB blank voltage to around 4.5

Software—advanced toolbox
Change language
Choose the type of data saved
Update
Software—one key export
U disk：
Kingston 8/16G
SanDisk 8/16G
Maxell 8/16G
FAT32
to reserve 4G
space.
Analyzer Info, software adjust Info, closed-reagent Info. Save to USB flash
disk, files named10000和10000_service, if the SN of the analyzer is 10000

Software—update
Create an upgrade USB drive

Unzip update.rar and copy the unzipped “update” directory to the root directory
of the USB drive.
Upgrade
Insert the USB drive into the USB port on the analyzer. Enter the Advanced
Toolbox and press[update] to upgrade the software
The upgrade process can be divided into two steps.
•Step 1: upgrade the guidance and operation system
• Step 2: upgrade the software components
When upgrading the boot and operation system, the operator will be prompted
to restart the analyzer between step 1 and step 2.
When upgrading the software components, the upgrade process will directly go
to step 2.

Software—communication
IP addresses of the PC and the analyzer shall be in the same

IP segment
Default IP of the analyzer：10.0.0.2
Software—communication （ Min dray Hem ato logy data
managem ent Communication so ft ware)
Port :5100
Analyzer’s IP
Select the actual analyzer model

the encoding mode of HL7 communication protocol is UTF-8,
and that of 15ID protocol is GBK

Software—communication （LIS ）
IP can
be
changed
contact your network administrator as to setting up IP address、subnet

mask and default gateway
Content
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Liquid system

Hardware—boards
 Main board(digital control board)

 Analog drive board
 Power supply board
 Indicator board
 Touch screen control board
 Laser control board
 Pre-amplifier board
 MAS&LAS PD board
 WAS PD board
 Liquid sensor board

Hardware—system o verview
Front panel Optical system

Touch
screen Laser PD
control
LED Screen
board
Touch screen Pre-
Laser drive
Indicator amplified
board
board board
Aurepert
Digital main board Analog driving board
HGB unit
Valve,
USB Motor floater
Power pump
supply
board Temp
Net Sensor hydraulic
control
Fluidic

Hardware—connection
Pinaster Master Analog&driver
Control Board Board
J1 Float
Ethernet J1
Communication J5 HGB
J81 J31
USB J2 J6 RBC
Optical
Analog Data J9 system
USB J3 J85 J4
Temperature
J10 sensor
BackLight J4 J11 Liq pressure
Supervise Signal sensor
LCD J86 J2 Optical
Data J16 J12 switch
Optical
Control Signal
J13 switch
Touch Screen J67 J77 J3
J14
Valve
Indicator J78 J15
J16 Pump
Start Key J99
J68 J17 Fan
P5V P12V J18 Fan

POWER Board
J19 Fan
D5V J1 Waste
J20
Pump
P12V/P24V J2 P5V
P12V J22 J21 Heat
A+12V/A-12V J3 P24V
J23 Motor
A+12V/A-12V
AC120V J4 J8 J24 Motor
AC120V

Hardware—Pin ster m ain contro l board
 Composition：Digital section, analog section

 Function:
 A/D conversion
Converts the analog signals to digital signals which can be processed by FPGA or
CPU.
 Data processing
The digital signals obtained from A/D samples are digitally filtered and save by
FPGA, then is transmitted to CPU for further processing, output the result.
 Peripheral interface implementation
The CPU module provides a platform for system software, interfaces for peripherals
 Control interface extension
Provides control logic and interface for LCD, SD card and touchscreen, etc

Hardware—Pinster main control board

Hardware—analog drive board
 composition：
 Power module
 Control module
 monitoring module
 Power drive module

 Power module
supplies power for all the electronic devices on the analog drive board, including both
input power from the analog drive board, and the power converted by the chip;
 Main control module
responsible for communicating with the control board and task scheduling;
 Monitoring module
collecting information such as temperature, pressure, voltage and fluid in the tubes;
 Power driving module
driving electric components such as motor, heater, fan, valves and pumps;
 RBC detection circuit
provides the RBC module with constant current source, RBC signal collection and
modulation;
 Modulation of optical signals and HGB signals.


Hardware—Power supply board
Name Socket No. Description
D5V J1
PIN1：GND
P12V
J2 PIN2、PIN3：5V
P24V
PIN1：GND
PIN2：12V
A+12V A- PIN3：24V
J3
12V PIN1、PIN4：GND
PIN2、PIN3、PIN5：-12V
AC120V J4
PIN6：12V
Alternating current
Hardware—In dicato r board
 Indicate the current status of the analyzer by means of sound and

light

Hardware—statu s o f in dicato r
Analyzer Status Indicator Comments
Ready Green light on Sequence is allowed
Running Green light flashing Sequence is being performed
Running with error Red light flashing An error is present and the
system is running
Stop with fault Red light on An error occurs and the
system is not running
No fault is present, but Yellow light on Initialization and standby
fluidics action is not status of sequence is not
allowed involved in the startup process
Enter/Exit standby status Yellow light flashing Enter/Exit standby status

Hardware—buzzer
Event Buzzer Prompts Comments
Startup completed One short beep Startup completed means the whole
startup process has been completed
and the analyzer is ready for operation
Open-vial aspiration completed Two short beeps
When count operation can not be One long beep If these screens have already
started in count related screens (including prompted, then the buzzer need not be
Sample Analysis, QC, Calibration, responded again.
Reproducibility, Carryover, Background,
Aging and Optical Gain Calibration
Count, etc.), press the aspirate key
Error Long intermittent Tap the touchscreen to stop the
beeps buzzer
Analyzer is ready One short beep Analyzer enters ready status from
other status
When the screen is black prompting stop beep If an error occurs during the
“Please power off the analyzer” shutdown process, the buzzer will
stop beeping when the screen is black
Hardware—touch screen drive board
 provides the interface between the touchscreen and the

control board
 converting the user's touch operation to identifiable signals for
main board.
The touch screen drive board needs to be adjusted before
use.

Hardware—Liqu id sensor board
 LED and Test point TP4

 output lower than 0.8V, the LED light is off, (have reagent)
 output higher than 2V, the LED light is on, (no reagent)

Hardware—optical boards
 Laser control board

 Pre-amplifier board
 MAS&LAS PD board
 WAS PD board

Hardware—Laser contro l board
 to provide stable and moderate-intensity driving current for laser

 Power modulation
 Laser driving current monitoring
 Laser power control.
 VR1:to adjust the power of the laser
Variable
Resistor
Control Laser driver Laser

(Closed-loop control) （HL6714G）
Voltage Laser current Power

supervise supervise PreProcess
A±12V
Laser Control
Board

Hardwar—Pre-am plifier board
 Power modulation
 I/V conversion
 Signal modulation.

Content
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Liquid system

Flu idic system —overview
 2 Analysis channel：WBC&HGB channel, RBC&PLT channel
Sample
RBC/PLT WBC/HGB
M-52D diluent
M-52 DIFF lyse M-52 LH lyse
Impedance
Laser scattering Laser scattering
\colorimetry
RBC/PLT/MCV Lymph、Mon、Eos、Neu
WBC/BASO/HGB

Fluidic system—overview
Flowchart
（WB-CBC+DIFF）

Peripheral Blood
Sample 20μl
Diluent 480μl
Fluidic system—overview
Sample Diluent
Ratio 1:25 500μl
Outside the analyzer
Inside the analyzer Aspirate 200 μl of sample

Flowchart Diluent Background WBC
358μl bath
（PD-CBC+DIFF） Aspirate 21.6 μl of
Sample Diluent secondary sample
Ratio 1:69.75
Variable temperature control
WBC
bath DIFF Lyse 1ml
Aspirate 380 μl of mixed fluid 36°
Sample WBC RBC

preparation LH Lyse 200μl bath Diluent 2.4ml bath
tube
WBC DIFF Sample WBC BASO Sample RBC Sample Diluent

Diluent Ratio 1:199.8 Diluent Ratio 1:234.3 Ratio 1:7750
Flow cell WBC bath

DIFF HGB RBC
Measurement Measurement bath
RBC/PLT
Sample preparation tube, flow cell cleaning Measurement
Flow cell
WBC/BASO
Measurement
Fluidic system—reagent consumption
Diluent DIFF LH lyse(ml) Probe

Mode (ml) lyse(ml) cleanser
（ml）
CD 27.5 1 0.2 0
WB
CBC 23.2 0 0.2 0
CD 27.5 1 0.2 0
Capillary_W
CBC 23.2 0 0.2 0
CD 27.6 1 0.2 0
Prediluted
CBC 22.9 0 0.2 0
Shutdown 60.9 1 0.2 2
Normal startup 65 1 0.2 0
Wake-up 1 3.7 0 0 0
Wake-up2 16.9 0 0 0
Wake-up3 65 3 0.2 0

Fluidic system—fluidic diagram
1 2 3 4 5 6
MRSZ/R05N01.291.02（2.0） REV ECN DESCRIPTION DESIGN
Add the information of LF, T76 and tubes in the optical assembly, Revise T3,
2.0 CR-3107-042 T10, T11, T18, T20, T22, T32, T44, T57, T66, T70, T71, T75, C18, C19, Yang Yijie
C20, C21
3.0 CR-3107-047 Add T80,J32 and revise T48,J15 Yang Yijie
SV08 C17 LF C22 C23

T19 T76 T20
A C14 DILUENT
T25 T49
CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
Preheat SV01
Bath T32
T26
T24
C27
J14-T48-J15-T80-J32 T47
T17
T77
SV02 DP2
J30-T78-J31
T18
Case
LH Reagent Bottle
WBC&HGB channel T79 SV07 C18
C19
T29
T71
J9
SV04
WBC RBC
RBC channel
T51
PV18
J25-T23-J26
B
C11 C10
J20-T63-J21 T34 T52
J22-T64-J23-T65-J24
Case T33 C6
J12-T45-J13
T72
T75
J10-T35-J11
Temperature Sensor
T54
J16-T59-J17
J18-T60-J19
C20
SV12
Isolation Chamber 1
Isolation Chamber 2
C21
T3
DIFF Reagent Bottle

SV16 SV14 C12 C13
T61 T62
T70
SV06
C5 SV13
T9
C4 T12 T13 T14 T37 T38

T39
T40
T55
C9
T15
T36
C3 SV11
T11 T16 C7
SV15
T41
C26 J5-T7-J6-T8
T10
Hydraulic
C Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
J3-T5
T2
T57 T58
T56
T42
J27-T22-J28
SV10 T67
C1 SV03
T43 T66
T1
SPB
Sampling/dispensing
J1-T4-J2
Diluent Tank
T73
VC
T74
SV09
T44
WASTE LP
Waste
Tank C25 C24
T69 T68 Confidential classification:
Confidential
SR CONFIDENTIAL
D
TITLE 3107 Pneumatic & Fluidics System Diagram
保密:此图及其全部知识产权(含著作权)归深圳迈瑞生物医疗电子股份有限公司所有。未经深圳迈瑞生物医疗电子股份有限公司预先书面许可,严禁出于任何目的,对 DOC No. A1-115-015670-00
此图的全部或部分内容(包括但不限于图中信息、数据、运算结果等)泄露、使用、拷贝或复制。
Classified documents, This set of drawing(s) and all it's intellectual property rights (including copyright) subsisting herein are property of DESIGN 杨一杰 REV. 3.0
Shenzhen Mindray Bio-medical Electronics Co.,Ltd. No disclosure,use,copies or reproductions should be made of this drawing or any part(s)
thereof for whatever purpose nor shall any information, data, calculations, or other contents contained in this drawing be disseminated without P.CODE 3107 SIZE A4
prior written permission of Shenzhen Mindray Bio-medical Electronics Co.,Ltd Software &Rev:
SHEET 1 OF 6
Microsoft visio 2003

Fluidic system—component
 Highly integrated air and hydraulic system
 17valves：1 high pressure (2-way)(V3);1pinch valve(V18)、1LVM(V11)
 1 syringe assembly (2 syringes，250ul、10ml）
 1 sampling assembly
 2 Electromagnetic Metering pumps（1ml、200ul）
 2 counting baths
 2 isolate chambers
 1 flow cell
 1 waster pump
 1 preheat bath assembly
 1 hydraulic pressure sensor
 1 check valve
 1 filter
 1 vacuum chamber

 VALVE

 Syringe Linkage
Syringe Linkage consist of a motor, a

driving assembly and two syringes
Name Specification Function

Small Used for quantitative aspiration, distribution and
Full scale
volume secondary aspiration of blood samples, and for injecting the
250μL
syringe sample into the flow cell for measurement
Used for quantitative addition of diluent to WBC and
Large RBC bath, wipe fluid supply, cleaning of interior and
Full scale
volume exterior of sample probe and reaction bath, forcing the
10mL
syringe sheath into the flow cell, cleaning of the flow cell and
sample preparation.
 Electromagnetic  Hydraulic pressure sensor

Metering Pump
symbol
symbol
Function： Function:
DP1：1ml，DIFF lyse adding Monitoring fluid pressure,
DP2：200ul，LH lyse adding give a alert signal when
abnormal or beyond settings.
 Preheat bath  Tem. Sensor for

diluent
symbol symbol
To heat DIFF lyse， Monitor Temp of dluent,

maintain the Temp in recommend a target Temp to
DIFF reaction bath(WBC preheat bath
bath)
 Fluidic temperature control
Item Preheat bath diluent

Target Refer to diluent
Temp
Alarm <target Temp -1.5 <10, >40
Temp >target Temp +3
Diluent
Temperature
Sensor
Reaction Bath
Temperature
J10 Sensor
PIN1 Temperature
Temperature
Analog Drive Switch
Switch
Preheat
Preheat
J21 assembly
assembly
Board PIN3

 Waste pump  Check valve
drain the wipe block, WBC bath, Define the flow direction of
RBC bath and vacuum chamber, DIFF lyse in case of reverse
create vacuum in the vacuum flow
chamber Attention : direction！

Fluidic system—component  Wipe block
 Sample probe
Aspirate and dispense the blood

sample and probe cleanser.
Note: the sample probe is flat-tipped Clean the sample probe and drain
with a side opening to ensure normal out the waste fluid while
aspiration in case that the tip touches cleaning the inner and outer of
the bottom of the sample tube. the probe

 Count reaction bath

 New structure
 Easy to maintenance
 WBC bath：for sample mixing ,reacting, HGB measuring,

provide diluted and reacted sample for DIFF\BASO counting
 RBC bath：
 50um aperture(improve the accuracy of PLT)
 Special design to reduce bubble in back bath
 Consist of aperture, front bath and back bath
 RBC diluting and mix and counting

 HGB assembly
 New design
 Convergent optical path
 Easy to Maintenance

 Vacuum chamber：maintain negative pressure for RBC counting.

Same with that of BC3000
 WBC/RBC Isolation chamber :provide an isolation air room to

cut off the internal or external interference

Cooperate with DP1 for DIFF Wipe block drainage ， vacuum
SV01 SV10
lyse suction /drainage pressure release
Control syringe switch to
Cooperate with DP2 for LH
SV02 SV11 sampling or sample preparation
lyse suction/ drainage
 Valve function
route
On/off between large and RBC bath to vacuum chamber
SV03 SV12
mall syringe on/off control for RBC counting
SV04 On/off the sheath flow SV13 RBC drainage
Control diluent adding to Rinse sample preparation pipe
SV05 SV14
RBC bath ,RBC bubbling
SV06 Bubbling for WBC bath SV15 WBC drainage
RBC bath rinse, cooperate
SV07 with SV09 to rinse the SV16 Floe cell drainage
wipe block
Control syringe suction
of diluent, cooperate Pinch valve for sample
with SV7/sv9 in preparation, cooperate with
SV08 PV18
dispensing diluent to RBC syringe controlling sample
back bath and wipe block preparation and rinse
，
cooperate with SV7 to
SV09
104 rinse the wipe block
Fluidic system—functioning and sequence
 Sampling/dispensing channel
SV08 C17 LF
T19 T76 T20
Function：
Diluent
T24
 Sampling 、HGB
T17
SV07
C3
Fluidic
pressure
SV11
RBC
measurement
T51
C26
 Rinse the outside of the

J5-T7-J6-T8
T10
sensor T53
C2
J4-T6 SV10 T52
C6
J3-T5
SP
T2
T57
J27-T22-J28
C1 SV03
 Rinse the inside of the

T1
SPB
T66
J1-T4-J2
T58
SV09
T43 SP
T44
VC
 Suck and dispense
SR Waste
LP probe cleanser
T68

Function：
 Sampling 、HGB
SV08 C17 LF
T19 T76 T20
diluent measurement
T24
T17
C3 RBC
SV07
SV11
SP
T51
C26 J5-T7-J6-T8
T10
T53
C2
J4-T6 SV10 T52
C6
J3-T5
T2
T57
SP
J27-T22-J28
C1 SV03

T1
SPB
T66
J1-T4-J2
T58
SV09
T43 probe cleanser
VC
T44
LP
SR
T68

Function：
SV08
T19
C17
T76
LF
T20  Sampling 、HGB
diluent measurement
T24
T17
SV07  Rinse the outside of the

C3 RBC
SV11
SP
T51
C26 J5-T7-J6-T8
T10

T53
C2
J4-T6 SV10 T52
C6
J3-T5
T2
T57
SP
J27-T22-J28
C1 SV03

T1
SPB
T66
J1-T4-J2
T58
SV09
T43 probe cleanser
VC
T44
LP
SR
T68

SV08 C17 LF
T19 T76 T20
diluent
T24
T17
SV07 Function：
 Sampling 、HGB
C3 RBC
SV11
T51
C26 J5-T7-J6-T8
T10
T53
C2
J4-T6 SV10 T52
measurement
C6
J3-T5
T2
T57
J27-T22-J28
C1 SV03
SP
T1
SPB
T66
J1-T4-J2

T58
T43
SV09
T44
VC SP
LP
SR
T68 probe cleanser

 WBC&HGB channel
SV08 C17 LF C14
T19 T76 T20 T25 T49
CV1
diluent J29 J7-T31-J8 T30
DIFF lyse
DP1
C27 Preheating T32
SV01
chamber
T77
C15
J30-T78-J31
T50
T26
T9 LH lyse
J14-T48-J15-T80-J32 T47
T17
SV02 DP2
SV04
WBC
Function：
J9
PV18
J25-T23-J26
J20-T63-J21
C11
外套
T34
C10
T33
 Inject 240ul diluent
J12-T45-J13
T75
J10-T35-J11
J22-T64-J23-T65-J24
 Dispense 15ul blood +

J16-T59-J17
SV16 SV14 C12 C13
SV06
C5
T61 T62
diluent 312ul
T9
C4 T12 T13 T14 T37 T38
 Suck diluted sample 12ul

C3 SV11
C9
T15
T11 T36
C26
T10
 Add 1ml DIFF lyse

T53 T16 C7
C2 J5-T7-J6-T8
SV15
T41
J4-T6
J3-T5
T2
T46
 Bubble mixing
C8
J27-T22-J28
C1 SV03
T42
T1
T66
SPB
J1-T4-J2
VC
Waste LP
SR
T69 T68

 WBC&HGB channel
SV08 C17 LF C14
T19 T76 T20 T25 T49
CV1
J29 J7-T31-J8 T30
diluent DIFF lyse
DP1
C27 Perheating SV01
T32
chamber
T77
C15
J30-T78-J31
T50
T26
T79
J14-T48-J15-T80-J32 T47 LH lyse
T17
SV02 DP2
SV04
WBC
J9
PV18
J25-T23-J26
J20-T63-J21
C11
T34
C10
Function：
T33
 Sample preparation 380ul

J12-T45-J13
J10-T35-J11
T75
J22-T64-J23-T65-J24
J16-T59-J17
 Adjust the position in the
SV16 SV14 C12 C13
T61 T62
SV06
C5
T9
C4 T12 T13 T14 T37 T38

C3 SV11
C9 tube
T15
T11 T36
Fluidic
C26
T10
 Add 200ul LH lyse

pressure T53 T16 C7
C2 sensor J5-T7-J6-T8
SV15
T41
J4-T6
J3-T5
T2
 Bubble mixing
T46
C8
J27-T22-J28
C1 SV03
T42
T1
T66
SPB
J1-T4-J2
VC
Waste LP
SR
T69 T68

 WBC&HGB channel
SV08 C17 LF C14
T19 T76 T20 T25 T49
CV1
diluent J29 J7-T31-J8 T30 DIFF lyse
DP1
C27 Preheating SV01
T32
chamber
T77
C15
J30-T78-J31
T50
T79
T26
LH lyse
J14-T48-J15-T80-J32 T47
T17
SV02 DP2
SV04
WBC
Function：
J9
PV18
J25-T23-J26
J20-T63-J21
C11
T75
T34
C10
T33
 To form the sheath flow
J12-T45-J13
J10-T35-J11
J22-T64-J23-T65-J24
 To boost the sample

J16-T59-J17
SV16 SV14 C12 C13
 DIFF count
T61 T62
SV06
C5
T9
C4 T12 T13 T14 T37 T38
 HGB measurement
C3 SV11
C9
T15
T11 T36
Fluidic
C26
T10
pressure T53 T16 C7

SV15
T41
J4-T6
J3-T5
T2
T46
C8
J27-T22-J28
C1 SV03
T42
T1
T66
SPB
J1-T4-J2
VC
Waste LP
SR
T69 T68

 WBC&HGB channel
SV08 C17 LF C14
T19 T76 T20 T25 T49
CV1
diluent J29 J7-T31-J8 T30 DIFF lyse
DP1
C27 Preheating SV01
T32
chamber
T77
C15
J30-T78-J31
T50
外套
T26
T79 LH lyse
J14-T48-J15-T80-J32 T47
T17
SV02 DP2
SV04
WBC
Function：
J9
PV18
J25-T23-J26
J20-T63-J21
C11
外套
T34
C10
T33
 Suck 156.3ul sample
J12-T45-J13
T75
J10-T35-J11
J22-T64-J23-T65-J24
from WBC bath

J16-T59-J17
 Rinse the flow cell
SV16 SV14 C12 C13
T61 隔隔 T62
SV06 离离
C5 室室
T9
C4 T12 T13 T14 1 2 T37 T38
 Rinse the route of sample

C3 SV11
C9
T15
T11 T36
C26
T10
液压传感器 T53 T16 C7

C2 J5-T7-J6-T8
SV15
preparation anf flush the
T41
J4-T6
J3-T5
T2
T46
C8
J27-T22-J28
C1 SV03
sample injector and flow
T42
T1
T66
SPB
cell
J1-T4-J2
VC
废液 LP
SR
T69 T68

 WBC&HGB channel
SV08
T19
C17
T76
LF
T20
CV1
T25
C14
T49 Function：
 Sample preparation
J29 J7-T31-J8 T30 DIFF lyse
diluent
Preheating DP1
C27 SV01
chamber T32
800ul
T77
C15
J30-T78-J31
T50
T79
T26
LH lyse
J14-T48-J15-T80-J32 T47
 To form sheath flow
T17
SV02 DP2
SV04
WBC
 To boost the sample

J9
PV18
J25-T23-J26
C11 C10
 BASO counting
J20-T63-J21 T34
T33
T75
J12-T45-J13
J10-T35-J11
J22-T64-J23-T65-J24
J16-T59-J17
SV16 SV14 C12
T61 T62
C13
 Rinse(same as DIFF)
SV06
C5  WBC bath drainage
T9
C4 T12 T13 T14 T37 T38
 Inject diluent to WBC

C3 SV11
C9
T15
T11 T36
Fluidic
C26
T10
pressure
T53 T16 C7
sensor J5-T7-J6-T8
C2 SV15
bath
T41
J4-T6
J3-T5
T2
T46
C8
J27-T22-J28
C1 SV03
T42
T1
T66
SPB
J1-T4-J2
VC
Waste LP
SR
T69 T68

 WBC&HGB channel
SV08 C17 LF C14
T19 T76 T20
diluent J29
CV1
J7-T31-J8
T25
T30
T49
DIFF lyse
Function：
 Rinse (as DIFF)
DP1
C27 Reheating SV01
T32
chamber
T77
C15
J30-T78-J31
 Rinse and drain out
T50
T79
T26
LH lyse
J14-T48-J15-T80-J32 T47
T17
SV04
WBC
SV02 DP2
WBC bath
J9
 Inject 2018ul diluent to
PV18
J25-T23-J26
C11 C10
J20-T63-J21 T34
T33
WBC bath as base
J12-T45-J13
T75
J10-T35-J11
J22-T64-J23-T65-J24
J16-T59-J17
SV16 SV14 C12 C13
solutioon
T61 T62
SV06
C5
T9
C4 T12 T13 T14 T37 T38

C3 SV11
C9
T15
T11 T36
Fluidic
C26
T10
pressure T53 T16 C7

SV15
T41
J4-T6
J3-T5
T2
T46
C8
J27-T22-J28
C1 SV03
T42
T1
T66
SPB
J1-T4-J2
VC
Waste LP
SR
T69 T68

 RBC&PLT channel
SV08 C17 LF
T19
Fuction ：
T76 T20
diluent
T21
 Inject diluent (1600ul)

T18
T24
SV05
SV07
T29
 Add diluted sample
T17
RBC
T51
(12ul) to RBC bath
T52
Temp. sensor J20-T63-J21 C11

C6
 Bubble mixing
T54
J18-T60-J19
 RBC、PLT counting
J10-T35-J11
SV12
J22-T64-J23-T65-J24
T3
C13
T62
SV13
SV14
C4 T37 T38
T39
C9
T40
T55
C3 SV11
T11 T36 C7
Fluidic T43
T41
C26 J5-T7-J6-T8
T10
pressure
T53
sensor C16
C2
J4-T6 C8
Transducer
J3-T5
T2
T56
T42
J27-T22-J28
T67
C1 SV03
T66
T1
SPB
J1-T4-J2
VC
SV09
T44 LP
Waste
T69 T68
SR

 RBC&PLT channel
SV08 C17 LF
T19
T76 T20
diluent
T21
Function：
T18
T24
SV05
SV07
 Rinse the RBC
T29
front bath
T17
RBC
T51
T52
C6
Temp.sensor J20-T63-J21 C11
T54
J18-T60-J19
J10-T35-J11
SV12
J22-T64-J23-T65-J24
T3
C13
T62
SV13
SV14
C4 T37 T38
T39
C9
T40
T55
C3 SV11
T11 T36 C7
Fluidic T43
T41
pressure C26 J5-T7-J6-T8
T10
sensor T53
C2 C16
J4-T6 C8
Transducer
J3-T5
T2
T56
T42
J27-T22-J28
T67
C1 SV03
T66
T1
SPB
J1-T4-J2
VC
SV09
T44 LP
Waste
T69 T68
SR

 RBC&PLT channel
SV08 C17 LF
Function：
T19 T76 T20
diluent
 Rinse the RBC
T24
back bath
T17
SV07
C3
Fluidic
SV11
RBC
 Inject diluent
T51
pressure C26 J5-T7-J6-T8
T10
C2
J4-T6
sensor T53
SV10 T52
(2560ul) to RBC
C6
bath as base
J3-T5
T2
T57
J27-T22-J28
C1 SV03
solution
T1
SPB
T66
J1-T4-J2
T58
SV09
T43  Release vacuum
VC
T44
LP
SR Waste
T68

Fluidic system—sequence in detail
 Measurement sequence in PD-CBC+DIFF mode

Measurement sequence in PD-CBC+DIFF mode is basically the same
with the sequence in WB-CBC+DIFF mode, except that:
Blood sample has been prediluted outside the analyzer,
200μL of prediluted blood is aspirated in the PD mode
 Measurement sequence in CBC mode
Compared with the sequence in CBC+DIFF mode, the measurement
sequence in CBC mode is basically the same except that:
Sample volume: 15μL in the WB-CBC+DIFF mode, 11.7μL in the WB-
CBC mode.(but for PD mode, CBC+DIFF and CBC mode are the same )
There is no actions related to DIFF measurement, including addition of DIFF
reagent, preparation of DIFF samples, DIFF measurement, and cleaning
process of the tubes and the flow cell after the DIFF measurement.

Fluidic system—sequence for maintenance
Maintenance with probe cleanser(shut down） SV08 C17 LF C22 C23
T19 T76 T20
The orange lines are the CV1

T25
C14
T49
DILUENT
T21 J29 J7-T31-J8 T30
locations soaked by the SV05

Preheat
T32
SV01
DP1
C15
T50
Bath
T26
T24
probe cleanser, C27
J14-T48-J15-T80-J32 T47
T17
T77
SV02 DP2
J30-T78-J31
T18
the blue lines are the Case
LH Reagent Bottle
T79 SV07 C18
C19
T29
locations passed by the
T71
J9
SV04
WBC RBC
probe cleanser
T51
PV18
J25-T23-J26
C11 C10
enhanced probe cleanser J20-T63-J21
Case
T34
T33
T52
C6
J22-T64-J23-T65-J24
J12-T45-J13
T72
T75
J10-T35-J11
Temperature Sensor
T54
J16-T59-J17
J18-T60-J19
maintenance sequence
Isolation Chamber 1
Isolation Chamber 1
C20
SV12
C21
DIFF Reagent Bottle

T3
will be called every 300 SV16 SV14 C12

T61 T62
C13
T70
SV06
C5 SV13
T9
times of sample C4 T12 T13 T14 T37 T38

T39
T40
T55
C9
T15
T36
measurement. C3
T11
SV11
T16 C7
SV15
The soaking time of
T41
C26
T10
Hydraulic
Sensor T53
C2 T46 C16
J5-T7-J6-T8 C8
J4-T6
enhanced maintenance is Transducer

J3-T5
T2
T57 T58
T56
T42
J27-T22-J28
SV10 T67
C1 SV03
6.67S T1
T43 T66
SPB
In the Maintenance
Diluent Tank
J1-T4-J2
T73
VC
screen, the probe
T74
SV09
T44
WASTE LP
Waste
cleanser maintenance is Tank C25 C24
T69 T68
defaulted as enhanced SR
probe cleanser
119
maintenance.
Fluidic system—sequence in detail
Starting up rinse
The fluidic actions on startup can be divided into three procedures:：
1. Initialization of fluidics components: initialization of sampling assembly and
syringe assembly; 1 metering pump action (without consumption of reagents);
creating vacuum and releasing vacuum.
2. Cleanup: including cleaning of all the tubes and components on the analyzer;
removing bubbles from the flow cell; discarding 1 ml of DIFF reagent and 0.2 ml
of LH reagent.
3. Background: with WB-CBC+DIFF measurement sequence
If the background fails, perform the cleanup again and measure the
background.
For startup after an abnormal shutdown, perform the cleanup twice.。
Initialization of fluidics components, cleaning up of the interior and exterior
surface of the sample probe while starting up after updating

Fluidic system—standby
 The instrument will enter standby status after idling for 15 to 30 minutes (defaulted as
15 minutes). After entering standby status, operations without fluidic actions can be
performed from the screen.
 Exit standby status 1: Standby for less than 1 hour.
Clean the exterior of the sample probe and the WBC bath and rebuild the
isolation bubble without consumption of lyse.
 Exit standby status 2: Standby for more than 1 hour and less than 5 hour.
 Clean the interior and exterior of the sample probe, the WBC bath, the RBC
bath, the sample supply tube and the flow cell, and rebuild the isolation bubble
without consumption of lyse.
 Exit standby status 3: Standby for not less than 5 hour.
The same as cleanup on startup, including cleaning of all the tubes and
components on the analyzer; removing bubbles from the flow cell; discarding 3 ml of
DIFF reagent to eliminate the effects of bubbles in the preheat bath tube; discarding
0.2 ml of LH reagent to eliminate the effects of crystal and bubbles.
目录
 Clinics
 Overview
 Principle
 Installation
 Software
 Hardware
 Optical system
 Liquid system

Mechanical system —overall layout
① 2-way valve
② 3-way valve
③ Syringe assemly
④ 1ml metering pump
⑤ microswitch
⑥ [aspirate] key
⑦ 200ulmetering pump
⑧ Sampling assembly
⑨ Fluidic pressure
sensor
 Front view of the main unit (cover opened)

① Analog drive board

② Main control board
③ Fan assembly
 Back view of the main unit (internal structure)

① Optical system
② Front cover
③ Reagent baffle
④ LH lyse
⑤ DIFF lyse
⑥ Net 、USB interface
⑦ Power supply
assembly
 Left view of the main unit (left

door removed)
① Fluid inlet/outlet
② Waste pump
③ Vacuum chamber
④ valve
⑤ LVM valve
⑥ Diluent Temp. sensor
⑦ Sampling assembly
⑧ Front cover
⑨ RBC bath
⑩ WBC bath
 Right view of the main unit (right door removed)

① Bath cap
② Pan head screw M3X6
③ Bath holder
④ Bath adjust holder
⑤ RBC bath
⑥ Aperture (D50um)
⑦ Front bath washer
⑧ RBC back bath
 RBC bath subassembly

① Bath cap
② Pan head screw M3X6
③ Bath holder
④ Bath adjust holder
⑤ WBC bath
⑥ HGB assembly
 WBC bath assembly

Mechanical system —disassem bling
RBC bath maintenance assembly

pipes are fixed to the holder

with cable tie
Winding pipe and

adapter should
be tied up with
cable tie Replace with specific pipe and
surround with winding pipe as
illustration in the figure

pipes are fixed to the holder

with cable tie
Winding pipe and

adapter should
be tied up with
cable tie Replace with specific pipe and
surround with winding pipe as
illustration in the figure

Disassembly rear plate of power

supply assembly

Mechanical system —adjustm ent o f b ath position

Mechanical system —adjustm ent o f b ath position
Quality Control
Operation
Operation content
Procedure QC Point QC Standard
Click on “Service → Sample Probe Debug”. Enter the
1.1
Debug screen as shown in Figure 1.1.
First, click on the “Initial position” so that the sample
1.2 probe is above the RBC bath, as shown in Figure 1.2.
Click on the “WBC bath”(or “RBC bath”) button shown in Fine adjust The sample
Figure 1.2. Click the “Middle position” button. Check if the the relative probe is
sample probe is basically in the center of the bath. If there position of basically in the
1.3 is any deviation, loosen the four screws shown in Figure the bath center of the
1.3 and slightly adjust the bath in left/right and front/back bath
directions so that the sample probe is in the center of the
bath. Then tighten one screw in each direction
Click on the “Initial position” button, then click “WBC Check if The sample
bath”(“RBC bath”) →“Down position” button. Check there the bath probe can not
is no interference with the bath as the sample probe position is contact with the
1.4
enters the bath. If the interference occurs, readjust the successfully bath。
bath position in accordance with step 1-3 and repeat this adjusted
step to check. Finally, tighten the 4 screws.
Note:
In Figure 1.1, all the buttons except the “Initialization” button will be grey before clicking on Initialization, the
three buttons in the “Sample probe horizontal position” column will be illuminated and available for use Only
after clicking on Initialization. The “Middle position” and “Down position” buttons will not be available until
clicking on the “WBC bath” or “RBC bath” button.

Mechanical system —m atters n eed atten tion
All parts of the analyzer are potential infectious, safety protection measures
should be taken during operation and maintenance
Reagents may irritate eyes, skin and mucous membrane. Operator who
contact such items as reagent in the laboratory, should comply with the
laboratory safety operating rules, and wear personal protective outfit (such as
laboratory protective clothing, gloves, etc.)
Once the skin is in contact with Reagent, wash immediately with plenty of
water, if necessary, please go to see a doctor; Once the reagent is splashed
into your eyes, rinse immediately with plenty of water, and treated by doctors
when removing some component with electrostatic sensitive sign, electrostatic
ring or static gloves and other protective equipment need to be equipped to
avoid damage to the parts
Choose the correct specifications and models of tube and screw
keep the original tube length. Any prolongation and shortening are strictly
forbidden.
Pay attention that the tube and cable could not bend or squeeze,
when reinstall, to prevent block or short circuit

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BC-5000&BC-5150 - Service Training Material - V1.0 - EN (REV)

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BC-5000/BC5150 service training

© 2013 Mindray Confidential

2 © 2013 Mindray Confidential

 Blood consists of 40-50% blood cell and 50-60% plasma.

3 © 2013 Mindray Confidential

Five groups of leukocyte

WBC 5-part proportion

Eosinophil 0.5 ～3%

4 © 2013 Mindray Confidential

 Morphology：size, shape, cytomembrane, cytoplasma, nucleus,

RBC WBC PLT

5 © 2013 Mindray Confidential

6 © 2013 Mindray Confidential

7 © 2013 Mindray Confidential

NEU EOS BASO LYMPH MONO

Cytoplasm Large more more less More than

Neutral Eosinophilic basophilic Azurophilic

Shape Fine Round or ellipse Differ in Differ in

Amount Much Much Less A few or

Color Pale violet Orange red or Royal purple Purple red

9 © 2013 Mindray Confidential

10 © 2013 Mindray Confidential

11 © 2013 Mindray Confidential

12 © 2013 Mindray Confidential

Monocytes percentage Mon% Hematocrit HCT

Platelet count PLT

Mean Platelet Volume MPV

Platelet-large cell ratio P-LCR

Platelet-large cell count P-LCC

13 © 2013 Mindray Confidential

Abnormal lymphocytes number ALY#

Abnormal lymphocytes Percentage ALY%

Large immature cells number LIC#

Large immature cells percentage LIC%

14 © 2013 Mindray Confidential

 Front of the analyzer

15 © 2013 Mindray Confidential

1、M-52D diluent inlet

 Back of the analyzer

17 © 2013 Mindray Confidential

item BC-5000 BC-5150

18 © 2013 Mindray Confidential

19 © 2013 Mindray Confidential

20 © 2013 Mindray Confidential

Reagent Name Type

Diluent M-52D DILUENT M-52D

M-52DIFF LYSE M-52DIFF

Probe cleanser PROBE CLEANSER /

Control : Mindray m ade

21 © 2013 Mindray Confidential

22 © 2013 Mindray Confidential

23 © 2013 Mindray Confidential

24 © 2013 Mindray Confidential

25 © 2013 Mindray Confidential

2ed Sheath flow 1st sheath flow

Blood sam ple

26 © 2013 Mindray Confidential

 Multi-angle laser scattering；

27 © 2013 Mindray Confidential

More difference in complexity

28 © 2013 Mindray Confidential

Lymph Mono Nue Eos Baso

29 © 2013 Mindray Confidential