Beruflich Dokumente
Kultur Dokumente
-Cells
are enclosed by a delicate membrane consisting of
protoplasm
Has a variety of physiological properties indicating cellular
functions
1. Irritability
2. Contractility
3. Conductivity
4. Absorption
5. Secretion and Excretion
6. Respiration
7. Growth and Reproduction
General Ultrastructure Of the Cell (LM)
Shape
Spherical in shape
Depends mainly on functional adaptations and partly on
the surface tension and viscosity of the protoplasm
Cytoplasmic microtubule- influence the shape of the cell
Size
The great majority of cells are visible only with the use of
the microscope since they are only a few microns in
diameter
Structure
Can be studied only with the aid of the microscope
1.
Small dense bodies of varying size and shape limited by a membrane and
containing a number of hydrolytic enzymes for intracellular digestion
Essential roles in cellular defense mechanisms
Its enzymes are collectively referred as “acid hydrolases”
Constitute an intracellular digestive system capable f breaking down
material originating both outside and inside the cell
Found in all cells except erythrocytes
6.
Self duplicating organelles that exhibit continuity from one cell generation
to the next
Double in number immediately before cell division
Prominent in mitosis
Essential for the formation of cilia and flagella
Serves as basal bodies and sites of epithelial cilia
- hairlike processes and numerous in epithelial cells of
the upper respiratory tract, parts of female and male reproductive tracts
and epyndymal lining the cavities of the CNS
- longer than cilia, show different undulating wave type
of movement
8.
Has similar structure with lysosomes but does not contain lysosomal
hydrolases
Contain several enzymes involved in production or destruction
Numerous in hepatic parenchymal cells and proximal convoluted tubules
9.
Visible only in EM
Consists of parallel arrays of cisternae showing annuli or circular fenestrae
at regular intervals along their length
Carry information from the nucleus to the cytoplasm
Seen in rapidly dividing cells
12.
Based on:
Number of layers
Simple/non-stratified-only one cell layer
Complex/stratified-more than one layer of cells
Shape of the cells on the free surface
Squamous
Columnar
* The first word in the name of an Epithelium
indicates the number of layers of cells while the
second word indicates the shape of the cells in
the free surface.
Classification and Distribution of Epithelial Tissues
1. Simple/non Stratified
1.1 Simple Squamous
1.1.1 Endothelium- lining of the blood vessel, lymph vessels and
cavities of the heart
1.1.2 Mesothelium- lining serous cavities like pleura, pericardium,
peritoneum and tunica vaginalis testis
1.1.3 Mesenchymal- lines the interior chamber of the eye, perilymph
spaces of the internal ear, subdural and sub arachnoid spaces
1.1.4 Flattened cells- lining the pulmonary alveoli, Bowman’s Capsule
1.2 Simple Columnar
Subdivided into:
1.2.1 Simple plain Columnar
1.2.1.1 Simple Plain Tall Columnar- mucosa of the stomach, small and large intestine,
gall bladder, bigger ducts of glands
1.2.1.2 Simple Plain Low columnar- smaller ducts of glands, some excretory tubules of
the kidney
1.2.1.3 Cuboidal- thyroid follicles, choroid plexus, pigmented epithelium of the retina
1.2.2 Simple modified Columnar
1.2.2.1 Simple Ciliated Columnar with:
Motile Cilia- uterus and oviducts of fallopian tube
Non-motile Cilia or Stereocilia- epididymis
1.2.2.2 Pyramidal/Glandular Epithelium
Serous Glandular- parotid gland and pancreas
Mucous Glandular- sublingual glands
1.2.2.3 Goblet Cell- found among cells of simple columnar epithelium of small and large
intestines and pseudostratified columnar epithelium of the respiratory tract
1.2.2.4 Neuroepithelium
Tastebuds
Organ of Corti-internal ear
Retina
Olfactory epithelium
1.2.2.5 Pigment Epithelium- retina
Simple Squamous Epithelium
Very thin, flat cells
Mosaic pattern
Attenuated cytoplasm with central bulging nucleus
Simple Cuboidal Epithelium
Row of square or rectangular profile
Nuclei tend to align at the same level in all of the cells
Box like/cube like
Simple Columnar Epithelium Simple Ciliated Columnar
A membrane composed of cylindrical cells
possessing an appreciable height aside
from length and width
Epithelium
Nuclei are at the same level and situated Possesses hairlike projection
nearer to the basal surface than the apical termed “cilia” on their distal end
surface Direction of Cilia is always towards
Associated with secretion or absorption the outside
Pyramidal or Glandular Epithelium
Narrow lumen of the acinus surrounded by Wider lumen surrounded by secreting cells
secreting cells
2. Complex or Stratified
2.1 Tall Columnar- innermost layer next to basement
membrane, cells are young with mitotic figures for replacement
of worn out cells, pigment granules are present in the cytoplasm
2.2 Polyhedral- with spherical dark staining nuclei becoming
vesicular as they reach the periphery, cells exhibit projections
giving a spiny appearance ”prickle cell layer” (stratum
spinosum)
2.3 Squamous/Flattened cells without nuclei- superficial layer
appearing like scales on the outer body surface, cells become
cornified or hardened, keratin prevents dehydration of
underlying cells, keratinized stratified squamous epithelium
2.4 Squamous with nuclei- innermost surface of the body, non-
keratinized stratified squamous epithelium, found in the epidermis
of the skin, lining of the oral cavity, epiglottis, esophagus and
Vagina
Pseudostratified Columnar Epithelium
Appears to be composed of
several layers of cells when in
reality, there is only one layer
All cells rest upon the basement
membrane but not all the cells
reach the surface
Cells vary in shape
Tall columnar
Fusiform/spheroidal-male urethra,
duct of parotid gland, trachea,
primary bronchi, auditory tube
Transitional Epithelium
Consist of many
layers(contracted stage)
Deepest layer- one or two
rows
Pyriform/pear shaped cells-
one to three rows
Superficial layer-
flattened/umbrella shaped
cells
Two layers(stretched)
Superficial layer- large
flattened squamous cells
Layer of cuboidal cells
Thin basal lamina
With degree of elasticity
Appear very thin when
organ is distended and
thicker when the organ is
collapsed
Found in urinary bladder
Connective Tissue
Type of tissue that is mesodermal in origin which
provides structural and metabolic support for
other tissues and organs found widely distributed
in the human body.
Widely dispersed system of cells fibers and
ground substance associated with muscle,
nerve, vasculature and all body organs.
Fewer cells are set apart due to abundant
intercellular substance or ground substance
containing fibers, except blood
Functions
Fibroblasts
Synthesize collagen and elastic fibers and glycosaminoglycan of the amorphous intercellular substance
Secrete procollagen molecules into the intercellular matrix
Macrophage(phagocytes,histiocytes)
Most abundant in richly vascularized areas
irregularly shaped cells, capable of amoeboid movement with pseudopodia
Important agents of defense
Act as scavengers because of their mobility and phagocytic activity engulfing extravasated blood cells,
bacteria, foreign bodies and dead cells
Play a fundamental role in development of immunity
Fat Cells(adipose or adipocytes)
Storage of neutral fats
Incapable of mitotic division
Can be stained with osmic acid
Mast Cells
The granules contain heparin(anticoagulant) and histamine(vasodilation and increases the
permeability of capillaries and small venules)
Actively involved in anaphylactic sensitivity reactions
Plasma Cells
Ovoid, irregularly shaped cells, smaller than macrophage but larger than lymphocytes
Nucleus are small and eccentrically placed
Major producer of antibodies
Pigment Cells
Resemble fibroblasts whose cytoplasm contain pigment granules(melanosomes) that never invade
the nucleus
Blood Leukocytes
White blood cells are frequently found in connective tissues
Lymphocytes- smallest of the free cells of connective tissue with spherical darkly staining nucleus,
they accumulate in chronic inflammation
Two types:
o T lymphocytes- long lived and responsible for initiating cell mediated immune response
o B lymphocytes- short lived which are capable of transforming into large immature cells when stimulated by
an antigen
Eosinophils- not numerous in connective tissue but abubdant in lactating breast and of the respiratory
and alimentary tracts
Nucleus are usually bilobed with spherical granules which are highly refractile, staining with acid dyes.
Accumulate in the blood and in the tissues in certain allergic, parasitic infections and subacute inflammatory
conditions
Contain slight amount histamine and comparison with mast cells
Neutrophils
Round cells with a segmented nucleus ranging from 3 to 4 lobes.
Granules are finer and neutropholic in staining
Monocytes
Rarely seen
Considered as phagocytes because they become very amoeboid and often show inclusions that stain
supravitally with neutral red.
Basophils
Contains granules similar in composition and function to mast cells
Types of connective tissues fibers
Most are derived from mesoderm except the neuroglia or supporting tissues
of the CNS which is derived from the ectoderm and the reticulum of the
thymus which are derived from endoderm.