ACETIC ACID TEST

Prepared by:
Dr. Herlie B. Ontoy, RN,MAN
 DEFINITION:
 It is a test for presence of albumin in
urine. TOXEMIA is a disorder during 3rd
trimester characterized by increase in
blood pressure, presence of edema, and
presence of excess protein in the urine.
 It is a test used to detect presence of

protein in urine.
PURPOSE:
 To detect presence of protein in

the urine
 To help evaluate and monitor

kidney function
 To help detect and diagnose early

kidney damage and disease

 IMPLEMENTATION
STEPS RATIONALE
 Fill the test tube  A fresh concentrated

2/3 of clear urine voided urine specimen

should be used.
 Done to avoid precipitate.
 Heat the upper
third of the test Too much heating of lower
tube. Lower test tube may results to
portion is not explosion
heated to serve
as control
 Implementation
STEPS RATIONALE
 If clouds forms, add  Cloudy urine is often
few drops of 5% due to phosphate
acetic acid. Heat precipitation in alkaline
again and allow urine. The phosphate
cooling. Read after 5 and Carbonates are re-
minutes dissolved when acetic
acid is added and
provided optimum Ph.
PRINCIPLE: Protein coagulates when
heated.

INTERPRETATIONS:
 If CLOUDINESS persist after 5 min, it

is due to the presence of albumin.

 If CLOUDINESS disappear, it is due

to the presence of PHOSPHATE.

 Nursing Consideration
 Follow strict standard precaution in
obtaining specimen.
 Collect specimen from the site with the

most viable and active microorganism

 Collect specimens in sterile containers

to avoid contamination
 BENEDICT’S TEST
 It is a test for presence of sugar in
urine. It is done in pregnant mothers to
determine diabetes.
 Is a reagent used as a test for the

presence of reducing sugar such as

glucose, lactose and fructose, but not
sucrose in a solution.
Purpose of Benedect’s
Test
 It can be used to test the presence
of glucose in urine; glucose found
to be present in urine is an
indication of diabetes
 Implementation
STEPS RATIONALE
1. Place 5 cc of 1. If its color changes
Benedict’s the solution is
solution in a clear contaminated
test tube. Heat, if
color changes,
discard.
Cont..
2. Add 8-10 drops of 2. The sugars that
urine and mix may occur in the
thoroughly urine can be
distinguished as the
basis of the test.
3. Boil for several 3. This is done to
minutes (2 mins) ensure adequate
results
 Interpretations
 Blue (-) = no  Sensitivity of the
sugar present test is
approximately 50-
80 mg of
glucose/100 ml.
 Green (+)= slight  It is usually = to
trace less than
50mg/100ml of
reducing sugar.
 Interpretation
 Yellow (++)= trace  Equivalent to 115-
550mg/100ml of
reducing sugar.
 Equivalent to 1400-
 Orange (+++)=
2000mg/100ml of
moderate amount
reducing sugar
 More than 2000-/
 RED (+++)= large 1000ml of reducing
amount sugar.