Burning Test for Proteins
Place a small amount of egg albumin on an
evaporating dish and apply heat gently.
Preparation of Egg Albumin
Sample
Separate the white from the yolk of an egg.
Place 150ml of distilled water in a 250ml
beaker. Add the egg white to the water while
stirring the mixture. Observe the appearance
of the mixture.
Filter the mixture and use the filtrate for the
following procedures.
Color Reactions and Proteins
Biuret Test
Place 3ml of egg albumin in a test tube. Add
3ml of 10% NaOH, then add a very dilute
solution of CuSO4 drop by drop, shaking the
mixture after each addition.
Millon’s Test
1. Place 1ml of egg albumin in a test tube.
Add 2 drops of Millon’s reagent. Heat in
boiling water bath for 10 minutes, then allow
to cool in running water.
2. Then add 4 drops of freshly prepared
0.1% NaNO2 and warm gently.
Xanthopreteic Test
1. Place 3ml of egg albumin in a test tube.
Add 3 drops of conc. HNO3.
2. Apply heat to the mixture
3. Cool and make solution slightly basic by
adding NH4OH solution.
Precipitation Reactions
By heat : Place 3ml of egg albumin in a test
tube and apply heat for a few minutes.
With Strong Mineral Acid : Place 2ml of
egg albumin in a test tube. Hold the tube in
an inclined position. Add 4ml conc. HNO3
slowly along the side of the test tube, (DO
NOT SHAKE). Observe the color at the
junction of the two layers.
With Salt of Heavy Metal : Place 2ml of
egg albumin in a test tube. Add 1% HgCl2
drop by drop with shaking. Count the
number of drops to produce a reaction.
Add an excess of the reagent. Note whether
the amount of precipitate is increased of
decreased.
With Alkaloid Reagents : Place 2ml of egg
albumin solution in a test tube. Add 10%
Tannic acid solution drop by drop shaking
after each addition. Count the number of
drops needed to produce a precipitate. Then
add an excess of the reagent.
Test for Sulfur. The Lead Acetate Test :
Place 1ml each of egg albumin solution and a
pinch of gelatin in separately labeled test
tube. Add 5 drops of 10% NaOH and 3 drops
of 5% lead acetate solution into each of the
test tubes. Shake and heat in boiling water
bath. Describe the color of the precipitate
formed.
Hopkin’s Cole Test : Place 1ml each of eff
albumin and 5% gelatin solution in
separately labeled test tubes. Add 5 drops of
Hopkin’s Cole reagent. Incline the test tube
carefully allow 1ml of concentrated H2SO4 to
slide down the side of the inclined test tube.
(DO NOT SHAKE). Take note the color at the
junction of two liquids.
 Burning Test for Proteins
Place a small amount of egg albumin on an
evaporating dish and apply heat gently.
Preparation of Egg Albumin
Sample
Separate the white from the yolk of an egg.
Place 150ml of distilled water in a 250ml
beaker. Add the egg white to the water while
stirring the mixture. Observe the appearance
of the mixture.
Filter the mixture and use the filtrate for the
following procedures.
Color Reactions and Proteins
Biuret Test
Place 3ml of egg albumin in a test tube. Add
3ml of 10% NaOH, then add a very dilute
solution of CuSO4 drop by drop, shaking the
mixture after each addition.
Millon’s Test
1. Place 1ml of egg albumin in a test tube.
Add 2 drops of Millon’s reagent. Heat in
boiling water bath for 10 minutes, then allow
to cool in running water.
2. Then add 4 drops of freshly prepared
0.1% NaNO2 and warm gently.
Xanthopreteic Test
1. Place 3ml of egg albumin in a test tube.
Add 3 drops of conc. HNO3.
2. Apply heat to the mixture
3. Cool and make solution slightly basic by
adding NH4OH solution.
Precipitation Reactions
By heat : Place 3ml of egg albumin in a test
tube and apply heat for a few minutes.
With Strong Mineral Acid : Place 2ml of
egg albumin in a test tube. Hold the tube in
an inclined position. Add 4ml conc. HNO3
slowly along the side of the test tube, (DO
NOT SHAKE). Observe the color at the
junction of the two layers.