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Objective of the paper

Biological background required in this work
Theoretical Basis of the work
Results
Conclusion
Future Scope of work

To determine the responsible gene(s) of a living

system which is causing a disorder in the
normal behavioral pattern of the living system.
The same system can be used for drug
design
The same system can be used to determine
which gene(s) has caused a spastic behavior of
a new born

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Rrotein
Replication
Transcription
Translation
Genes
Micro-array data

Source of micro-array dataset

www.ncbi.nlm.gov.in in GEO database

Dataset used in this work is of Burkholderia

Pseudomalli in time series format

Our approach in this paper

mat[5289,47] is formed keeping the genes at the

rows and time instants at the columns.

mat[i,j] represents the value of ith gene at jth

instant of time.

We are going to find out the idelity Matrix

out of this dataset

0ll elements of column k are subtracted from the

corresponding elements of column k+1

Result is stored in column k where k=1, 2, 46

This reduces the dimension of the original matrix

to 5289 by 46

0verage value of the expressions of each gene

is calculated

The average is subtracted from each gene

expression

Then absolute value corresponding to each of

the gene in the matrix is calculated. This is the
idelity Matrix.

Then every expression is compared with a

predefined threshold value such that if the
value of expression is less than zero, then it is
set to zero otherwise it is kept as it is.

Discard genes that have all zeros as their values

Thus the 5249 by 46 matrix is reduced to 311 by 46

matrix.

These 311 no. of genes are identified as the set of

responsible genes

560, 2598, 1868, 934, 1277, 2552, 3123, 962, 129, 991, 3380, 4784, 4216, 1552, 2914,
1581, 3190, 60, 502, 3, 111, 4149, 3075, 935, 4355, 3004, 1221, 3379, 1161, 4220, 3272,
3528, 2722, 3887, 4417, 2868, 2056, 1648, 2572, 3002, 1193, 3977, 5114, 1994, 1980, 447,
549, 1165, 2409, 1431, 3373, 5273, 1964, 1751, 1752, 195, 4144, 263, 3738, 2687, 573,
4215, 2980, 1249, 3523, 4505, 2347, 4803, 4959, 3905, 2960, 3494, 987, 2011, 2751, 4967,
3940, 225, 1985, 956, 3001, 1058, 5107, 2430, 5235, 943, 314, 4294, 1410, 98, 4804, 1141,
62, 1400, 2682, 2563, 955, 2162, 4327, 2855, 2071, 4916, 4041, 862, 1211, 3886, 2215,
2216, 4915, 1140, 1972, 778, 3884, 1928, 3809, 3802, 958, 1612, 22, 3128, 37, 3536, 875,
1370, 173, 1804, 3166, 2406, 3526, 3124, 338, 2453, 3455, 2956, 3315, 2744, 2953, 5189,
334, 771, 198, 2922, 4421, 3140, 4418, 3801, 2374, 4515, 4031, 1280, 584, 1198, 1960,
2957, 1248, 1243, 2292, 4078, 5246, 271, 1733, 4423, 5027, 2954, 1456, 1444, 208, 937,
330, 1554, 376, 5061, 4433, 2170, 780, 2752, 2647, 2021, 2955, 4311, 4227, 3262, 4027,
4950, 4802, 3432, 2101, 3593, 2576, 1132, 1993, 4911, 2725, 5251, 503, 897, 4310, 4035,
3941, 2454, 1349, 4492, 3646, 2837, 3617, 1129, 4223, 864, 3656, 2641, 4913, 1137, 5134,
601, 3168, 2583, 1169, 365, 400, 4937, 161, 1170, 3867, 1105, 4325, 870, 2455, 1266,
3709, 509, 2865, 1199, 3397, 787, 3389, 4652, 4332, 4744, 1210, 2944, 4240, 5257, 1647,
2207, 2634, 1116, 3482, 924, 1959, 4395, 4918, 59, 2845, 2982, 4696, 3731, 1508, 4077,
2472, 3016, 1799, 3694, 777, 1973, 3161, 3122, 4987, 4922, 3530, 2139, 2635, 1187, 4782,
1450, 4135, 5243, 4351, 826, 3060, 2714, 1038, 4514, 275, 5253, 1414, 3041, 488, 3077,
4434, 640, 3167, 1124, 4725, 781, 980, 685, 4289, 3370, 258, 3383, 3919, 1428, 2792,
5245, 1592, 1171, 3960, 2847, 1618, 4071, 74.

Dere out of 5289 by 47 original dataset we found

that 311 by 46 are the responsible gene sets for the
desired activity of our interest in Burkholderia
Pseudomalli.
From the set of responsible genes, gene regulatory
network can be found out which plays a vital role
in drug design and genetic disorder.
Further research can be carried out to modify this
work in the field of metabolic pathway
engineering.

Thank you