CRYPTOCOCCUS NEOFORMANS

Presentation subtitle

SEJARAH
Sanfelice 1894 : peach juice Italy (Sanfelice, 1894 ) Busse dan Buschke ,1895 : cryptococcosis : chronic ulcer tibia Verse 1914 : cryptococcal meningitis Stoddart dan Cutler 1916 : patologi CNS kapsul polisakarida

Struktur sel Cryptococcus Neoformans (Sumber : Simmer dan Secko, 2009)

MIKROSKOPIS
Bulat kecil bertunas ragi berkapsul Hifa dan pseudohifa invivo invitro Sel ragi berdiameter 5-10

Tunas tunggal atau multipel.

Ukuran kapsul bervariasi

(Perfect, 2010).

SIKLUS HIDUP

. Diferensiasi morfologis pada CN (Sumber : Lin, 2009)

Komplek spesies CN
Cryptococcus Serotipe Neoformans Cryptococcus A var. grubii Neoformans AD hybrid D var. neoformans Cryptococcus B Gattii C Representasi klinik 95% <5% <1% Filamentasi Perkawinan Ya Ya Ya Filamentasi sendiri Tidak Ya Tidak Pseudohifa Ya Ya Tidak

 C. gattii (serotypes B and C) is most often isolated from trees

C. neoformans (both serotypes A, D, and AD hybrids) is more often isolated from bird guano

 Basidiospora/ ragi kering inhalasi paru granuloma (bentuk dormant) imunosupresi menyebar

CRYPTOCOCCUS GRUBII

From left to right: Encapsulated yeast cells Sexual spores from Serotype A mating Encapsulated yeast and sexual spores on defined media Basidia head bearing four spores at distinct positions Hyphae with a clamp cell, yeast cells and sexual spore chains (Source: Rajesh Velagapudi, and Joseph Heitman, M.D, PhD., Duke University)

C VAR NEOFORMANS

Velagapudi et al., 2009

C GATTII

Velagapudi et al., 2009

Perbandingan
Velagapudi et al., 2009

Perbandingan

Comparison of morphologies and spore sizes. Spores produced by mating, fruiting, or diploid self-filamentous C. neoformans strains were analyzed by SEM. A comparative profiling of the spores from H99 and KN99a serotype A mating (a), JEC21 and JEC20a serotype D mating (b), and monokaryotic fruiting of serotype D strain XL280 (c) and spores produced by self-filamentous diploid (aAA) strain KN2B5#19 (d) is shown.
Velagapudi et al., 2009