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Quick guide focusing on mathematical understanding (other parts not discussed in any length)
+
= [].
d[] ,
Here,
d[]
is also known as
= 1 [].
Kd
The formation of the ES complex can also be described as the association of the enzyme and substrate. This has a reverse reaction which we havent yet discussed, the dissociation of the ES complex into individual enzyme and substrate molecules
(We wont discuss the decay of ES into E & P here). Change in [ES] includes two terms: one positive, for the formation of the complex (a 2nd order reaction), and one negative, for the dissociation of the complex (a 1st order reaction).
= 1 1 []
Kd cont
Kd is a way of expressing the balance of these reactions, defined as the rate constant of dissociation (k-1 above) divided by the rate constant of association (k1) How is this value experimentally determined? At equilibrium, there is no overall change in concentration of ES over time, because the forward and reverse reactions are in balance. = 1 1 = 0 (at equilibrium). So 1 = 1
Rearrange to get 1 /1 =
[] []
then
TST, cont.
expands upon and includes the transition state ( ) in the reaction equation. This gives us another way to express the velocity of the reaction: v = [ ]. We can also express the equilibrium constant (not a Kd) of the part of the reaction: = /[]
1
The second page titled Transition State Theory- the meaning of the
Now, lets look at a similar.
1 = This is a formulation of the Arrhenius equation. A is not an amount in this equation. Its something called the pre-exponential factor or frequence factor. Has to do with number of collisions of molecules in solution of reaction. This value is usually defined experimentally.
1 + 1
= (by definition).
= +
( ][ )[] []