In vitro Assessment of Antimicrobial Activity of some Ornamental Flowers

Presented by: Bidisha Ghosh 8th Sem, B.Tech, Biotechnology, SITM

Under the supervision of: Mr. Neeraj Mishra Asst. Prof. & Head (Dept. of Biotechnology), SITM

Infectious disease & traditional drug problems

Leading cause of premature deaths, killing almost 50,000 people/day

Just 4yrs after the start of mass-producing penicillin in 1943 resistant microbes began appearing

Development of resistance to common antibiotics in all major pathogens are reported These negative health trends necessitated a search for new antimicrobial substances from other sources including plants.

Use of flowers as medicine

50% of pharmaceuticals originate from plants Most plants contain bioactive compounds that have good antimicrobial activity Plant antimicrobials are not associated with side effects Some flowers with reported antimicrobial activity: Chrysanthemum Jasmine Poppy Rosemary Thyme

Objective of project
Evaluating antimicrobial activity of water & alcohol extracts of 4 flowers against 5 microorganism by Agar Well Diffusion Method.

Flowers used
Cyme rose (Rosa indica) Lotus (Nelumbo nucifera)

Orange Marigold (Tagetes erecta)

Yellow Red Marigold (Tagetes patula)

Preparation of flower extracts Water extract

5 g flower + 100 ml dist. water

Ran in soxhlet (4 hrs at 100OC)

Alcohol extract

5 g flower + 100 ml alcohol

agitated for 8 hrs in a wrist-action shaker

alcohol evaporated without heat & residue mixed with 100 ml of dist water

Microorganisms analyzed
Bacillus subtilis Staphylococcus aureus

Escherichia coli

Pseudomonas aeruginosa

Candida albicans

Analysis of antimicrobial activity

Method: Agar Well Diffusion Method Media: Muller Hinton agar Parameter: Zone of Inhibition
Measuring the inhibition zone

Antimicrobial activity against Staphylococcus aureus

Water extract

Alcohol extract

Graphical representation of antimicrobial activity with water extract

12
inhibition zone(in mm)

10 8 6 4 2 0
Bacillus subtilis Staphylococcus aureus Escherichia coli Pseudomonas aeruginosa Tagetes patula Candida albicans

Rosa indica

Nelumbo nucifera

Tagetes erecta

Control

Orange Marigold showed activity against all five microorganisms Rose gave the highest inhibition. Candida albicans showed the highest resistance & was susceptible only to Orange Marigold & Yellow Red Marigold.

Graphical representation of antimicrobial activity with alcohol extract

12 inhibition zone (in mm) 10 8 6 4 2 0 Bacillus subtilis Rosa indica Staphylococcus aureus Nelumbo nucifera Escherichia coli Tagetes erecta Pseudomonas aeruginosa Candida albicans Control

Tagetes patula

Yellow Red Marigold showed very high activity against all five organism Rose also showed strong inhibiting capability Most resistant microorganism was again Candida albicans

Conclusion
Flower extracts have great potential as antimicrobial compounds Rosa indica & Tagetes patula showed maximum antimicrobial activity These flowers can be used to discover bioactive compounds that may serve as leads for development of new pharmaceuticals

Acknowledgements
Mr. Neeraj Mishra, Asst. Prof. & Head of the Dept., Biotechnology, SITM. Dr. Vinod Bihari, Director of Department of Biotechnology All the faculty of the Dept., Biotechnology, SITM. My parents.

Thank You