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Activity 3:

Transport Processes
Postlab Discussion
DIFFUSION AND MOLECULAR
WEIGHT: Part 1

What are formed upon the reaction of HCl


and NH4OH?

HCl + NH4OH NH4Cl + H2O


DIFFUSION AND MOLECULAR
WEIGHT: Part 2
1. Which solution has diffused the
farthest, congo red, KMnO4, or
methylene blue?

MW:
Congo red = 697
KMnO4 = 158
Methylene blue = 319
DIFFUSION AND MOLECULAR
WEIGHT: Part 2
1. How does molecular weight affect the
rate of diffusion?

Rate of diffusion is inversely proportional to


molecular weight.
DIFFUSION AND TEMPERATURE
1. Why is KMnO4 used in this experiment?
IONS:
Potassium = K+
Permanganate = MnO4-
 Because it is a salt that easily dissolves in
water.
 It dissolves in water to give deep purple
solutions
DIFFUSION AND TEMPERATURE
2. Describe the distribution of KMnO4 in
each beaker after 30 minutes.
DIFFUSION AND TEMPERATURE
3. What is the effect of temperature on
the rate of diffusion?

 An increase in temperature increases the


rate of diffusion, because diffusion is
dependent on the kinetic (thermal) energy
of molecules.
DIFFUSION AND MOLECULAR
SIZE
1. Where did you observe a color change,
in the beaker or cellulose sac? How
would you explain the color change?

 Color change occurred inside the sac.


 This is due to the entry of IKI.
DIFFUSION AND MOLECULAR
SIZE
2. Which substance has smaller molecules,
starch or iodine?

 Iodine (IKI)
 Because a change of color occurred
inside the bag, indicating the entry of IKI
into the bag.
DIFFUSION AND MOLECULAR
SIZE
3. In what way is the cellulose / dialysis sac
similar to the cell membrane

 It is semipermeable.
OSMOSIS AND THE RBC
1. Describe the shape of the RBC seen in
thethree slides. Draw your observations.
DIFFUSION AND MOLECULAR
SIZE
2. Which solution is HYPEROSMOTIC
compared to the RBC?

 5% salt, because it has a higher solute


concentration.
 Solution in slide = 95% H2O
 Solution in blood = 99.1% H2O
DIFFUSION AND MOLECULAR
SIZE
3. Which solution is HYPOOSMOTIC
compared to the RBC?

 dH2O, because it doesn’t have salt


dissolved in it.
 Solution in slide = 100% H2O
 Solution in blood = 99.1% H2O
DIFFUSION AND MOLECULAR
SIZE
4. Which solution is ISOOSMOTIC
compared to the RBC?

 0.9% salt, because it has the same


concentration of salt as the RBC.
 Solution in slide = 99.1% H2O
 Solution in blood = 99.1% H2O
MEASURING THE RATE OF
OSMOSIS
20
18
16
14
12
10
8
6
4
2
0
DIFFUSION AND MOLECULAR
SIZE
1. What is the relationship of osmosis with
the concentration of sucrose

 Direct relationship
DIFFUSION AND MOLECULAR
SIZE
2. Explain the results in sac # 5.

 The sac became lighter.


 Water moved from the sac to the beaker
due to the hyperosmoticity of the solution
outside the sac.
END.

Thank you.