1 Mobile Phase Reservoirs 2 Degasser 3 Pump

Figure 1: Bottle Head Assembly Figure 2: Degasser Parts & Accessories Figure 3: Pump Capillaries Figure 4: Pump Modules Figure 5: Pump Valve Assemblies Figure 6: Pump Assembly Part 1 Figure 7 : Pump Assembly Part 2

4 HiP ALS Injector

Figure 8: Injector Parts & Vials Figure 9: Injection Valve Assembly

5 Column Compartment 6 Detector

Figure 10: Column Compartment Figure 11: Diode Array Detector Figure 12: Flow Cell Assembly

Agilent 1200 HPLC

Description
1 2 3 4 5 6 7 8 Solvent tubing, 5 m Ferrules with lock ring (10 pack) Tube screw (10 pack) Bottle cap with 3 hole insert Inlet filter adaptor Solvent inlet filter, 20 um Tubing for needle wash Bottle head assembly (includes 1 ea of parts 1-6)

Reference
C1 A2 A3 C4 A4 B18 C2 C7

Figure 1

Bottle Head Assembly

Description
1 2 3 4 5 6 7 8 Vacuum chamber Solvent tubing kit (4 tubes) Tube clip Syringe adaptor Syringe Mounting tool Tube cutter Tube cutter blades

Reference
E1 E2 A5 A6 A1 A7 A10 A11

Figure 2

Degasser Parts & Accessories

Description
1 2 3 4 5 6 Solvent mixer standard & short Solvent selection valve PEEK Adaptor Plug for SSV Bracket for solvent mixer Gradient valve

Reference
B4-B5 E3 A8 A9 E4 E5

Figure 3

Pump Modules

Description
1 2 3 4 5 SS capillary, 150x0.17 mm (pressure sensor to dampener) SS capillary, 105x0.17 mm (mixer to dampener/purge valve) Mixing capillary assembly Restriction capillary SS capillary, 400x0.17 mm (pump to injector)

Reference
E6 E7 E8 E9 B1

Figure 4

Pump Capillaries

Description
1 2 3 4 5 6 7 8 Active inlet valve AIV cartridge Outlet ball valve Ball valve sieve (10 pack) Gold seal Outlet cap (4 pack) Purge valve Purge frit (5 pack)

Reference
E10 A16 C5 A12 A23 A29 C6 A35

Figure 5

Pump Valve Assemblies

Description
1 2 3 4 5 6 7 8 9 10 Tubing for seal wash 5 m Silicon tubing adaptor Support ring with wash seal Seal wash gasket (6 pack) Wash seal Seal keeper (2 pack) Pump seal (2 pack) Wash seal insert tool Pump seal insert tool Restriction Capillary for seal wear-in procedure

Reference
C3 A13 E11 A14 A15 E12 A17 A18 A19 A36

Figure 6

Pump Assembly Part 1

Description
1 2 3 4 5 6 7 8 Purge valve holder (binary pumps only) Sapphire piston Peristaltic pump with silicone tubing Plunger housing with springs Pump chamber housing Adapter (binary pumps only) Absorber capillary (binary pumps only) Lubricant (apply to piston base)

Reference
E13 E14 E15 E16 E17 E18 E19 A20

Figure 7

Pump Assembly Part 2

Description
1 2 3 4 5 6 7 8 9 Loop capillary Needle assembly standard & low delay volume Needle seat 0.12 or 0.17 mm id Needle seat capillary 0.12 or 0.17 mm id Screw cap blue & green Standard HPLC vial, clear, screw top Conical HPLC vial, clear, screw top Fixed insert HPLC vial, clear, screw top Vial plate 54 vial, 96 well, & 96 well plate cover

Reference
B15 B19-B20 B23-B24 B25-B26 D1-D2 D3 D4 D5 D6-D8

Figure 8

Injector Parts & Vials

Description
1 2 3 4 5 6 7 8 9

Reference

Long nut fitting E20 SS capillary, 160x0.25 or 160x0.17 mm (valve to metering device) B2-B3 Plastic fitting/plug A21 SS capillary, 340x0.12 or 380x0.17 mm (valve to column) B6-B7 Waste tubing B8 Isolation seal B27 Rotor seal B28 Stator face (attached to stator head on models G1367C/D) B29 Stator head E21/E31

Figure 9

Injection Valve Assembly

Description
1 2 3 4 5 6 7 8 9 10 11 12

Reference

SS capillary, 70x0.12 or 90x0.17 mm (heat exchanger to column) B9-B10 Carrier for heat exchanger (includes 3 screws) B21 Heat exchanger long-down B30 Heat exchanger long-up B31 SS capillary, 50x0.12 or 150x0.17 mm male/female B11-B12 Adjustable fitting A22 Column fitting with ferrules (10 of each piece) A24-A28 ZDV union A30 Blanking nut A31 Column clip A32 SS capillary, 340x0.12 or 380x0.17 mm (column to detector) B6-B7 Cooler B22

Figure 10

Column Compartment

Description
1 2 3 4 5 6 7

Reference
C16 B13-B14 C8-C10 B16-B17 A33-A34 C11-C13 C14

Deuterium lamp SS capillary, 700x0.17 or 500x0.12 mm (detector to waste/MS) Flow cell standard, semi-micro, & micro DAD heat exchanger capillary - 0.12 or 0.17mm id PEEK fitting short & long PEEK tubing 0.18, 0.25, or 0.5 mm id Waste tubing corrugated

Figure 11

Diode Array Detector

Description
1 2 3 4 5 6 Front gasket (Teflon, 1.3 mm hole) Back gasket (Teflon, 1.8 mm hole) Quartz window Window holder Compression washer Spring washers (10 pack)

Reference
E22 E23 E24 E25 E26 E27

Figure 12

Flow Cell Assembly