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INTRODUCTION
The human somatic cell contains 46 (23 pairs) chromosomes. The total length of the DNA in a cell is about 2 meters. The question is how is all this packed into a single nucleus of 10 microns diameter ?????
INTRODUCTION
How The expression level of different genes vary with spatial and temporal distribution ???????
INTRODUCTION
The eukaryotic genome is organized into repeating disc shaped nucleosomal units composed of histones and their associated DNA. Nucleosomes are recognized highly dynamic units through which the eukaryotic genome can be regulated.
Genomic regulation
Nucleosome strings. Chambon and colleagues used trypsin to remove histone H1 from chromatin isolated from chicken red blood cells, revealing a beads-on-a-string structure. The bar represents 500 nm.
The number of regions that deform the externel ring ranges from 5 to 7.
The average diameter dimensions shown are calculated from chromatin arrays folded in 1.6 mM MgCl2.
The most abundant proteins of the chromosome scaold are DNA topoisomerase II (topo II) and ScII, which is a member of the SMC (stable maintenance of chromosomes) family.