David H. Spodick 1 Abstract Recent capability to enter the normal effusion-free pericardi- um has expanded intrapericardial therapy which was formerly restricted to patients with effusive pericardial disease, to intra- pericardial treatment of cardiac diseases of all kinds. It is now possible to deposit in the intact pericardium a variety of ther- apeutic agents targeting the myocardium, valves, conduction system and even the endocardium. In addition to such specific agents, the unique microphysiology of the pericardial meso- thelium provides investigators with 2 entirely new applica- Mikrophysiologie des Perikards: Das Substrat fr intraperikardiale therapeutische Anwendungen Zusammenfassung Die intraperikardiale Therapie hat die Mglichkeit erffnet, sich Zugang ins Perikard zu verschaffen, auch wenn kein Perikarderguss vorliegt. Diese Mglichkeit stand frher nur fr Patienten mit Perikarderguss zur Verfgung. Es ist heute mglich, in das intakte Perikard eine Reihe von thera- peutischen Substanzen einzubringen, deren Angriffspunkte Myokard, Herzklappen, Reizleitungssystem und sogar das Endokard sein knnen. Fr diese Therapie bietet die Mikro- physiologie des Perikardbeutels, ein einzigartiges, weitgehend abgeschlossenes System, ideale Voraussetzungen. Das Meso- thel verfgt ber Enzyme und Rezeptoren, zum Beispiel ber die Zyklooxigenase, Prostazyklinsynthase und Lipoxigenase, tions of intrapericardial therapy: 1. supplementing substances like prostanoids and a variety of immune factors, and 2. stimu- lating pericardial production of such products of metabolism, e. g., superfusion of the normal pericardium by non-steroidal antiinflammatory agents to stimulate prostanoid production with a variety of effects including possible inhibition of coro- nary thrombosis. Continuing research and development should determine the precise roles of these new applications in human medicine. das Endothelin, Sekretionsmechanismen fr Komplement- faktoren und Rezeptoren fr Fibronolytika. So ist in Zukunft gut vorstellbar, dass in intaktes oder entzndetes Perikard Substanzen wie Prostanoide und immunologische Faktoren (Zytokine und Antizytokine) eingebracht werden, um Stimulatoren fr die Produktion nicht steroidaler antiinflammatorischer Substanzen oder die Prostanoidproduktion zu frdern oder um eine Koronar- thrombose zu inhibieren. Dies ist nur ein Ausschnitt mg- licher Angriffspunkte zur intraperikardialen Anwendung am Menschen. Key Words: Pericardium Pericardial microphysiology Intrapericardial therapeutics Schlsselwrter: Perikard Perikardiale Mikrophysiologie Intraperikardiale Therapeutika 1 Worcester Medical Center/Saint Vincent Hospital, Medical School, University of Massachusetts, Worcester, MA, USA. Pericardial macroanatomy and microanatomy [1] are uniquely configured to serve its macrophysiology and microphysiology [2]. Traditionally, intrapericardial therapy has been introduced when the macrophysiology has been affected by excessive pericardial fluid, permit- ting easy access not only for drainage but also to intro- duce specific medications (Table 1). Intrapericardial therapy in the absence of excessive fluid is aimed at di- rect and semi-direct treatment of heart disease, provid- ing at least 2 applications [1]: introduction into the nor- mal pericardium of antiarrhythmic, antiinflammatory, oxygen and NO-supplying agents and angiogenic and other agents (Table 2) [2]. Pericardial microphysiology extends the applications of intrapericardial therapy to Urban & Vogel 2000 Herz 720 Herz 25 2000 Nr. 8 Urban & Vogel Spodick DH, et al. Microphysiology of the Pericardium 721 Herz 25 2000 Nr. 8 Urban & Vogel agents that will stimulate favorable products of pericar- dial microphysiology and block or neutralize unfavor- able products of pericardial microphysiology. Pericar- dial microphysiology can be outlined as follows. Outline of the Microphysiology of the Normal Pericardium 2 Servomechanisms Pericardial servomechanisms contribute to mechanical and perhaps membranous functions of the pericardium. 1. Neuroreceptors in the epicardium and the fibrosa that detect lung inflation and may operate via the vagus nerves to alter blood pressure and heart rate. 2. Sympathetic efferents: these do the opposite of the va- gal effects. 3. Mechanoreceptors sensitive to changes in ventricular stretch determined by ventricular volume and trans- mural pressure. Pericardial dysfunction is sensed by mechanoreceptors with phrenic afferents that appear to monitor beat-to-beat changes in cardiac volume. Mechanoreceptors with unmyelinated fibers signal myocardial tension and appear to reflexly match con- traction strength with peripheral resistance, e. g., dur- ing exercise. (Some of these receptors output can contract the spleen and lower blood pressure.) 4. Chemoreceptors sensitive to substances in the peri- cardial fluid. (These actually include hybrid mecha- noreceptors responding to digitalis glycosides and perhaps contributing to bradycardic effects.) Normal Pericardial Fluid Normally, there are 15 to 30 ml of serous fluid in the per- icardial sac, mainly an ultrafiltrate of plasma that may include some overflow of myocardial interstitial fluid as well as myocardial lymph drainage. 1. Protein concentration: lower than in plasma but with a relatively high albumin component. 2. Electrolyte concentration: as predicted for a plasma ultrafiltrate. Mesothelial Metabolic Activity The mesothelium has cyclooxygenase, prostacyclin syn- thethase and lipoxygenase activities: 1. Prostanoids: Prostaglandin E2, eicosanoids and pros- tacyclin are continually released by the entire pericar- dial mesothelium into the pericardial cavity in re- sponse to pericardial stretching, to increases in myocardial work and loading and to hypoxia [3, 4]. They are additionally stimulated by angiotensin II and bradykinin. Pericardial prostacyclin may also potentiate the chronotropic and algesic properties of bradykinin, particularly during angina and perhaps also the pain of pericardial inflammation. Prostacy- clin is also a potent inhibitor of platelet aggregates and may inhibit thrombosis in the major coronary 2 Modified from [14]. Therapy Targets (pericardial disease) Fibrinolytic Blood, hemopericardium Streptokinase/streptodornase Pyopericardium, Hemopyopericardium Antineoplastics Malignant tissue Sclerotherapy Persistent effusions Corticosteroids Inflammation: general, vasculitic, immunopathic Antibiotics (generally Specific infections unnecessary) Table 1 Established intrapericardial therapy via pericardial effusions (modi- fied from [15]). Tabelle 1 Etablierte intraperikardiale Therapie ber perikardiale Effusionen (modifiziert nach [15]). Therapy Targets (cardiac disease) Gene Vessels: mesothelium, conducting tissues Angiogenic Vessels Apolipoproitein A-Milano Vessels: intima, adventitia Nitric oxide Vessels: lumen; walls Donors/precursor (L-arginine) Platelets Voltage sensitive Damaged (depolarized) cells Hypothermic Damaged/ischemic myocardium Oxygen Damaged/ischemic myocardium Antiarrhythmic Myocardium/conducting tissues Procainamide Amiodarone L-arginine (via nitric oxide) Antiarrhythmic/antibody Myosin of damaged cells Calcium-avid Increased cell calcium Table 2 Intrapericardial therapy via normal peridardium (modified from [16]). Tabelle 2 Intraperikardiale Therapie ber normales Perikard (modifiziert nach [16]). Spodick DH, et al. Microphysiology of the Pericardium 722 Herz 25 2000 Nr. 8 Urban & Vogel vessels as well as clotting when there is blood in the per- icardium. These prostanoids can alter pericardial sym- pathetic neurotransmission and myocardial contractil- ity and modulate the caliber and tone of the underlying vessels (i. e., direct vasodilation by prostaglandin and in- directly by opposing coronary spasm). Increased peri- cardial prostaglandins also inhibit efferent sympathetic effects acting as modulators of sympathetic neurotran- smission with multiple effects on cardiac electrophysiol- ogy [5], possibly including reduction of reperfusion-in- duced arrhythmias. 2. Endothelin: Mesothelial synthesis and release, in- creased by angiotensin II stimulation, may act as a vasoconstrictor. 3. Defensive Immunologic Constituents: Small amounts of complement (C3, C4, CH 50). Other immune fac- tors, myocardial cellular enzymes and related compo- nents are released into the pericardial fluid, neces- sarily transiting the visceral pericardium (unless damaged). 4. Fibrinolytic Activity: The intact mesothelial serosa itself opposes both intrapericardial clotting and for- mation of adhesions via its fibrinolytic activity. (Yet, after any kind of pericardial injury, including physical and chemical trauma, pericardial fibrinolytic activa- tor activity decreases.) Lubricant Function Reducing friction during the cardiac cycle: 1. generation of normal pericardial fluid, and 2. phospholipid surfactants capable of reducing friction between otherwise hydrophilic surfaces consistent with the classical theory of boundary lubrication [6], 3. cell-to-cell interaction between pericardial mesothe- lial cells and ventricular myocytes (so far demonstrat- ed in vitro) may have a role in the complex scheme of pericardial microphysiology. Intrapericardial Therapeutics and Pericardial Microphysiology To take advantage of pericardial microphysiology, intra- pericardial therapeutics would be aimed at a) stimulat- ing or supplementing the favorable effects of pericardial metabolites (e. g. prostanoids, prostacyclin synthetase, cyclooxygenase, lipoxygenase and antithrombotic sub- stances) and b) blocking or neutralizing potentially harmful substances like endothelin (unless vasocon- striction were, for some reason, to be desirable). The material in Tables 1 and 2 is self-explanatory. Established therapies via the enlarged pericardial space during pericardial effusion (more related to the macro- physiology of the pericardium) targets pericardial dis- eases. Intrapericardial therapy via the normal pericardi- um, as yet experimental, targets cardiac diseases [710]. Intrapericardial therapy utilizing pericardial micro- physiology (see outline) would target both pericardial and cardiac disease based on 2 approaches: 1. stimulat- ing the pericardial mesothelium to produce specific sub- stances in larger than normal amounts, and 2. either supplementing or blocking products of pericardial mi- crophysiology. Presumably intrapericardial deposition of therapeutic agents in large amounts would parallel the results of the procedures in Table 2 in the sense that systemic effects would be minimal or at least undetect- able so that large concentrations of appropriate agents could be delivered in preparations for long duration of delivery according to indication. A great deal of investi- gation will be necessary to bring these to practical clini- cal usage, including meticulous determination of risk- benefit ratios. Prostanoids produced by the pericardial mesotheli- um, for example, can alter pericardial sympathetic neu- rotransmission and myocardial contractility. They may modulate the caliber and tone of the underlying coro- nary vessels (i. e., direct vasodilation by prostaglandin and indirectly by opposing coronary spasm). Their pro- duction by the pericardium may be stimulated by non- steroidal antiinflammatory agents (NSAIDs), particu- larly the cyclooxygenase inhibitors delivered directly upon the epicardium [3, 11]. Inhibitors of efferent sym- pathetic neurotransmission in addition to eicosanoids affect cardiac electrophysiology and may reduce reper- fusion-induced arrhythmias [12]. Prostacyclin is also a potent inhibitor of platelet aggregates and may inhibit thrombosis within the major coronary vessels as well as clotting when there is blood in the pericardium. In the latter case, increased prostacyclin production would supplement the fibrinolytic activity of intact mesothelial serosa [2]. Prostanoids are also stimulated by angiotensin II and bradykinin. Since pericardial prostacyclin, generat- Spodick DH, et al. Microphysiology of the Pericardium 723 Herz 25 2000 Nr. 8 Urban & Vogel ed during hypoxia and work may potentiate the chrono- tropic and algesic properties of bradykinin, particularly during angina and perhaps also the pain of pericardial inflammation, bradykinin would not appear to be a practical clinical stimulant. On the other hand, angio- tensin II, if devoid of unwanted systemic effects, may be worth investigating as a stimulant; however, it also in- creases mesothelial synthesis and release of endothelin. During hemopericardium of any etiology there is a strong potential for adhesions and ultimate constriction, particularly when the fibrinolytic activity of the intact mesothelial serosa is reduced by injury (physical and chemical trauma as well as inflammation can decrease pericardial fibrinolytic activator activity [2]). Here, sup- plementation by antithrombotic agents, as already prac- ticed under some circumstances, may be desirable after drainage of blood and pericardial lavage. Striking success in animals (Table 2) for example, prevention of acute and chronic ischemia and of coro- nary atherosclerosis by NO donors [13] urgently calls for extensive and intensive investigative effects with intrapericardial therapy in human beings. References 1. Spodick DH. Normal pericardial macrophysiology. In: Spodick DH. The pericardium a comprehensive textbook. New York: Dekker, 1997:1823. 2. Spodick DH. Microphysiology of the normal pericardium. In: Spo- dick DH. The pericardium a comprehensive textbook. New York: Dekker, 1997:236. 3. Nolan RD, Dusting GJ, Jakubowski J, et al. The pericardium as a source of prostacyclin in the dog, ox and rat. Prostaglandins 1982;24:887902. 4. Dusting GJ, Nolan RD. Stimulation of prostacyclin release from epicardium of anaesthetized dogs. Br J Pharmacol 1981;74:55362. 5. Miyazaki T, Pride HP, Zipes DP. Prostaglandins in the pericardial fluid modulate neural regulation of cardiac electrophysiological properties. Circ Res 1990;66:16375. 6. Hills BA, Butler BD. Phospholipids identified on the pericardium and their ability to impart boundary lubrication. Ann Biomed Eng 1985;13:57386. 7. Seferovic PM, Ristic AD, Maksimovic R, et al. Initial clinical experi- ence with Perducer device: promising new tool in the diagnosis and treatment of pericardial disease. Clin Cardiol 1999;22:Suppl I:I305. 8. Macris MP, Igo S. Minimally invasive access to the normal pericar- dium: initial clinical experience with a novel device. Clin Cardiol 1999;22:Suppl I:I369. 9. Verrier RL, Waxman S, Lovett EG, et al. Transatrial access to the normal pericardial space. Circulation 1998;98:23313. 10. Spodick DH. Microphysiology of the pericardium in relation to intrapericardial therapeutics and diagnostics. Clin Cardiol 1999; 22:Suppl I:I23. 11. Herman AG, Claeys M, Moncada S, et al. Biosynthesis of prostacy- clin (PG12) and 12L-hydroxy-5,8,10, 10-eicosatetraenoic acid (HETE) by pericardium, peritoneum and aorta of the rabbit. Prostaglan- dins 1979;18:43953. 12. Zipes DP. Targeted drug therapy. Circulation 1990;81:113941. 13. March KL, Woody M, Mahdi K, et al. Efficient in vivo catheter- based pericardial gene transfer medicated by adenoviral vestors. Clin Cardiol 1999;22:Suppl I:I239. 14. Spodick DH. Physiology of the normal pericardium: functions of the pericardium. In: Spodick DH. The pericardium: a comprehen- sive textbook. New York: Dekker, 1997:156. 15. Spodick DH. Pericardial effusion and hydropericardium. In: Spo- dick DH. The pericardium: a comprehensive textbook. New York: Dekker, 1997:12652. 16. Spodick DH. Intrapericardial therapeutics and diagnostics. Am J Cardiol 2000;85:10124. Address for Correspondence: David H. Spodick, MD, DSc, FACC, Professor of Medicine, Director of Cardiovascular Fellowship Training, Worcester Medical Center/Saint Vincent Hospital, University of Massachusetts Medical School, 20 Worcester Center Blvd., Worcester, MA 01608, USA, Phone (+1/508) 363-6162, Fax -225, e-mail: DHSPOD@WMC.com Reproducedwith permission of thecopyright owner. Further reproductionprohibited without permission.