Hasil Pengukuran Nikotin 26 Juli 2023

Metode Spektrofotometri UV
Alat Implen NanoDrop T81334
Panjang Gelombang max 260 nm
Pelarut ddH2O
1. Kurva Standar Nikotin

Konsentrasi
Rata-rata
no nikotin standar absorbans
Absobansi
(ppm)

1 0.00 0.000
2 0.00 0.000 0.000
3 0.00 0.000
4 5.00 0.080 KURVA STANDAR
5 5.00 0.081 0.085
6 5.00 0.093
7 20.00 0.429 1.2

Absobansi pada Lamda = 260 nm

8 20.00 0.456 0.447 1
9 20.00 0.456
0.8
10 30.00 0.606
0.6
11 30.00 0.609 0.609
0.4 y = 0.0203x + 0.0067
12 30.00 0.613
0.2 R² = 0.9925
13 40.00 0.843
14 40.00 0.871 0.864 0
0 10 20 30 40 50 60
15 40.00 0.877
Konsentrasi Nikotin Standar (ppm)
16 50.00 0.978
17 50.00 0.973 0.976
Rata-rata Absobansi Linear (Rata-rata Absobansi )
18 50.00 0.976

KADAR NIKOTIN
KADAR NIKOTIN
FAKTOR FAKTOR SETELAH [Nikotin] rata-rata
NO KODE SAMPEL ASORBANSI SEBELUM
PENGENCERAN 1 PENGENCERAN 2 PENGENCERAN (ppm)
PENGENCERA (ppm)
(ppm)
1.1_1 100 1 0.391 18.93 1893
1 1.1_2 100 1 0.406 19.67 1967 1970
1.1_3 100 1 0.423 20.51 2051
1.2_1 100 1 0.500 24.30 2430
2 1.2_2 100 1 0.486 23.61 2361 2397
1.2_3 100 1 0.494 24.00 2400
1.3_1 100 1 0.874 42.72 4272
3 1.3_2 100 1 0.880 43.02 4302 4300
1.3_3 100 1 0.885 43.27 4327
1.4_1 100 1 0.600 29.23 2923
4 1.4_2 100 1 0.612 29.82 2982 2995
1.4_3 100 1 0.632 30.80 3080
1.5_1 100 1 0.900 44.00 4400
5 1.5_2 100 1 0.913 44.65 4465 4465
1.5_3 100 1 0.926 45.29 4529
2.1_1 100 1 0.174 8.24 824
6 2.1_2 100 1 0.191 9.08 908 870
2.1_3 100 1 0.185 8.78 878
2.2_1 33 1 0.674 32.87 1085
7 2.2_2 33 1 0.689 33.61 1109 1102
2.2_3 33 1 0.690 33.66 1111
2.3_1 50 1 0.593 28.88 1444
8 2.3_2 50 1 0.600 29.23 1461 1451
2.3_3 50 1 0.594 28.93 1447
2.4_1 50 10 0.253 12.13 6067
9 2.4_2 50 10 0.264 12.67 6337 6272
2.4_3 50 10 0.267 12.82 6411
 KADAR NIKOTIN
KADAR NIKOTIN
FAKTOR FAKTOR SETELAH [Nikotin] rata-rata
NO KODE SAMPEL ASORBANSI SEBELUM
PENGENCERAN 1 PENGENCERAN 2 PENGENCERAN (ppm)
PENGENCERA (ppm)
(ppm)
2.5_1 50 1 0.170 8.04 402
10 2.5_2 50 1 0.183 8.68 434 421
2.5_3 50 1 0.180 8.54 427
3.1_1 50 10 0.222 10.61 5303
11 3.1_2 50 10 0.257 12.33 6165 5878
3.1_3 50 10 0.257 12.33 6165
3.2_1 50 10 0.362 17.50 8751
12 3.2_2 50 10 0.383 18.54 9268 9055
3.2_3 50 10 0.378 18.29 9145
WHIPS_1 50 10 0.230 11.00 5500
13 WHIPS_2 50 10 0.253 12.13 6067 5886
WHIPS_3 50 10 0.254 12.18 6091

FOTO SAMPEL