Tnsrumental mdhods o Analysis Unit-

GP101T
Devivaizohon Techniqus in GC Dr. Parjanya Shukla
&
Dr. M.P.Singh Clases
xCas chromatoqraphy but powiblu tachniquu for
is
Aoparah om o vdlaHl compounds which are tarmally
stabl
duwing prOUK
x Som unchona qrobs NH, OH, NH, SH and cooH
CTaka pro.blem in GC Seporakan.
So Kay nud to be derivahzed
DeiniHon- Derivaizahom i h proua d chumically
modidying o Combound
to produu 0 nuo combound which has þroperkes tKak
aT Swtab.lu fo onalysis sing GGC.

x Th chumical shudunu od Ki compound xemaun hi somu

just modi izu ke dunchonal qroub q Muacing compaunds

Derivahzah m i nud1d in GG, HPLG, UV-Visibu Sprchoscapy eh.

Why DaTivahgabn
w
) ncrzas1 volahlity
Clininak m o palo groups uiku o, NH &SH
DYivab^abm tovgets, 0,S, N VP Jun tchon al grops.

) Incaau detectabilty

) Inast Stability.
4)T6 duu adsovphon opolor 60mplus on achva Aufau d
Column wall and solid Subpout
 Dr. Parjanya Shukla
&
Dr. M.P. Singh Claseo

SilyJahom ALkylabon Aoylahon Chiral

divahzabm.
a) Silylohoo
Machoniam TKin prous produus silyl derivahvs
which ova mo1 volaklu , moTA tKarmally stablu
uplaus ochv hydrogen oit Ti mdhy! Silyl graups
cH
cH3
Sompl-: H
+
CH3 Si-X Somplu -o-Si - x
H CH
cH
44 x =u hat i
hrimthyl chorosilonu cH3
Sompl - 0-Si -CH3 +HX

cH
Tri mahylsily! darivabv

Th 1ase o ochuity d funchonal graub touwords Ailylahon

oows R ordr
AlcoholPhenol Coboxy) >Amins Amids/bydroxy!
Advontaqss
Ability to silylake a widu vavishy o Compounds
(i Lorge numbr d ilylohing uagents available
Hexo mhyl disila zons (Hmos) Trimhylchlorosilanu(rm)
Timahylailylimida2ol (ms), Bishymahysilyloutomidu
(8sA),Bisimahlsilylmfluoroadkamids (BSTFA)
P-muhyl hrimhylsilyliroatlamidh (MSTFR) a.
Cii) Easily Praborid.
2
 Disodvontages -
Silloion uagen 0 moiskurs bonsihve. Dr. Parjanya Shakla

(Should stond in HighB A1alud containeTa) Dr. W. P. Singh Classes

i) Must w opToi orgonic bolwnt
) Alkylakon:-
x Alkylahm duces molu culor polari
u by uploung
aciva hydrogan wih on alky! group
Thus Huagents o ustd to modily compounds wik audic
hydoqo bko corborylic, oudic and phenols.

K COoH+ Ph ChaX RCOOCH9 Ph + HX.

Somu (ommm uggen ed are -

DialkylaulaBt, Diazoalkalus, Penlafluorobenayl bromidt

Benzylbromid, Bromo riluoride and Tehahulylammmium
hydroxide
Advantaqps
Ci °oid Honqe alkylaHon xuagents avalabl.

cio Kaochion condihon may vay

OnD domu xtackon can bi donu in aguuous nudium

Civ) Alkylahm derivah'ves 0T genuyally stabla

Disadyantaqas
c Cmitad to omints and auddic hydroxyls.

i) Raaqents ort oftan toxic

3)Acylakon- Dr. Parjanya Shukla
&
xAcylabm uduus R þolonity omino, bydroxyl, pr. M.1. SnghClases
and hio gsoups
n combarision to Ailylahm uagent
h ayloking uagenh torga highy þolar, mulhunchonal
Compaunds Auch as COTbohydrakes and amino adds.
omu Commm agen arl -

Juoroeyimidazolus, fuoTrinahd Anbydid,

N-Mahy-bis (rifluoroacelamid1) mBTeA), Pantolluoro.ban20oy
chlovid
chlorid (PPBCI) and Pentoluoroproponol (PFPoH)

CHa 0 CO CO CHa+ HOR (H)

CH3 CH OCOR + HOCO CH3

Advontaqp
c Addikon o Kaloqenated CaTbons incruasud dekechubibhy by ECD.
di) Dorivakves ore hydro.lyhcally stablh.
ii Tnuraas Agsihivi by addinq moluadar wight
(iv) Auylabon can bi d a diat tab to ackivate
COTboxylic audsprior to ostanjicaiam.
Disadvantages-
ti Acylahon derivahives can b1 di}|iult to prapara.
ii Reacbom produc offen nud to be emoved befoTA analyais.
) Atylahom) uagents ar moistur saniktve.
CIV) Raoqents ar hazardows and odovous.

By-Dr. Mahendro Pratap Singh

4) Chira Derivalizabon -
Dr. Parjanya Shukla

xThea
9royuagenBs
and targel one spuic funchenal Dr. W.P. Singh Clases

Toduu ndivi dual diasteviomaT oach onanhuomuY.

x
Thuss a sepavalad J-
Sopnaban on an opkeally achvs stahonary bha.

hsparabam o diaslavaomavic deuivahives Kat can bu

Brporaked on a non- stainary phasa
Keogents N- usd
tridluro auhl-L- þroby) chlorid (TP)
Mahu chloroforma (me)