Inshumunta Mahods_d Analysis 8P7oLT

Unil-
Advantages, Disadvantages and Applicabns
AChromdtograbhy "

Vr. Parjanya Shukla

&
Dr. M.P. 3ingh Clases

Advantoqos
)Caskous and Volahilu compounds con b eaily schovatad
and analy20d.

() Bok lualitahve and Quanhtahve onalysis can bs dons

)Tnshrument usud aTimplu, inexpensive and uggad
4)The tachniqu Can b id for xulint anabysis
as operahon otnarumanls and calculahim is simpl
5) suquires Jous omount o sompl (in ul)
So known as micro mehod.

6) Sonsitiviky o tachnique is quita higb

Compounds can be detectad u to porh per million (bbm)
aTh prT bilion (bbA)
By-Dr.Mahndro Aratap Sihqh Evin pitogqrama (1o"g9)

) MoT Kon 50 Organic compounds can be onaly2sd

by Ris techni qus
(Raxolvinga potoer is oxhamaly hig
S Combounds tKaB aT dihiaultto onalyzad b
otker tochniques con easily analy2ed GC.
Disadvantaqes
www Dr. Parjanya Shukla
&
() Sampl should bu volailu or vapoTi2ablu. (o Dr. M.P. SunghCaes
complata di'smbubonm b/w M-P. and S.P.
)To vapoviga a comþound 4 neded to be kaate d a
elovatad temprature
so sompb should bi Karmostaba.

(Lorge amount o ga IA ussd as mobil phaso and

Vcan not be uycld.
4) Packing Column i v dfiult
6)
(5) uquva Jong Hnu and operakura oih good knowbdge.

Abblicakons
) QualitahveApayas
xwalitahve anglysis by GC. can be donu by

a) Colloching olutad vopouTs TOm colump

hen ubjchd Ram to apeuic techní qus Jsku
mas Apectroscoby, U.V. 1R NMR et. ,

cb Compaing otenHon im volumu oAambl wih standoyd

(for Column low xate, ogas d temp oq column kubt canstont)

somp and Atondard compounds undar idenkical condi bons

gives Damu stusalubom timu indicahos both compaunds
0TA Aame.
C)Suuontitakv AnalyAiA Dr.Pardanya Shukla

uanitabva analyain by gas chromaBoqraphy Dr. M.P.3bngh Clases

dapends upon the sige paak
Hioht (H) or Ki avsa d ho peak (A)

4 is Auppoxed tRak K orsa undar tku þaak

is
roporhonal to t tonunabon odutad AubaBanta

Hrao undr cunva o pzak (A) = Hx WV

Gohur H is kight paak
Wy oidh peak a kalf hight
AcSambb) tsomblu)
AGt) C Atd)
whuut
AtAambl) Ptak o6amplu
ar20
AtAhd) Paak o0rea standard)
C tAompla) Comunhabon o Bomple
Cd) Conunabon eAtandatd
usponae actor

PhomouhicaAPblicakons
i) Qualily cmkol and Analysi o dug produck bk-
anhibioHch, Antiviral, onàusthehs , Audabva and hypnoics h

di) Asay d drugs pavity data minabon.

i) In datar mining Ki laval d druga and matabalita
in body luiids ke - plasma, SQYuM , uins at

(iV) TdanHicabon and dakaminabon o falky adds.

) lsolabm and id1nhiicabon o mixturs
o þlant
exacts, carbohydrokes, volahih oilb, amino audsDr.PartanyaShuila
ipids, protins, vitomins, lavowyA, toJauront, er r. M. P. Sngh loas

() MiscaDonuous Applicahons_
0) has chvomaBogvaphy is widaly wad in anaais
tilizs, sulhber, cOAmshCk,panumus, þahro.lum
roduch aod produch o
tor analyAis oday produch Jik milk, buter, chss eh.
) In environmuntal atudies.

(iv) T0r dataehion o staroids tken by ahJakos during

intnabonad sport compahi'tions.

By-Dr. Mohandu Ahatap Sing