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Nuclear Magnetic
Resonance Spectroscopy
Introduction
NMR is the single most widely used
physical technique because its wide
application.
It is used to study a wide variety of nuclei:
1H
13C
15N
19F
31P
spin
=>
Nuclear Spin
. ) ( '
%&' #
.0'12
+# - /
A nucleus with an odd atomic number or an odd mass number has a nuclear spin.
The spinning charged nucleus generates a magnetic field.
=>
Dr. USAMA El-AYAAN
8
8
Magnetic Shielding
If all protons absorbed the same amount of
energy in a given magnetic field, not much
information could be obtained.
But protons are surrounded by electrons
that shield them from the external field.
Circulating electrons create an induced
magnetic field that opposes the external
magnetic field.
Dr. USAMA El-AYAAN
10
Magnetic Shielding
K9 DEF / (= - HF
(= 4E 0 L?
.NMR
H M' 6H
4
.%:O / - )E/4 %' N2
4E
11
Shielded Protons
/
4E B %9 H - ( %&-) / R SBK
- W
& C # ? Q %' ( %&-)
. 2
/
4E
Magnetic field strength must be increased for a shielded proton to flip at the same frequency.
12
Protons in a Molecule
Depending on their chemical
environment, protons in a molecule
are shielded by different amounts.
13
=>
14
15
Deshielding
Shielding
A/B %&-
E/B 0H
16
CH3
H3C
Si CH3
CH3
17
Chemical Shift
Measured in parts per million.
Ratio of shift downfield from TMS (Hz) to
total spectrometer frequency (Hz).
Same value for 60, 100, or 300 MHz
machine.
Called the delta scale.
18
CH3
CH3 Si CH3
:I> F0& E
@KL *
M 7
'
.9(!*
CH3
Highly shielded
protons appear
way upfield.
TMS
shift in Hz
downfield
Chemists originally
thought no other
compound would
come at a higher
field than TMS.
19
h
F; 6H
9 %F; 6: K 0M 62 O4
:NMR h 0&4 -
^
YC: %9 (&O
4E
. Z 0 ; A K
0M 62 .1
26.5 oC ^(O _&: ` F 0Z
.2
. C ` G&O 0) # 26.
/ %&-( B %( eO) f #) B % /
H - ): G4 `4
42 .3
J %&- (< J TMS %FO hF #;P .0& 6
& (F
FO
. (ppm) &
C/2 )- E %
E& C4) O
- &'1
:
4E& C Higher frequencies
= larger shifts in Hz.
TMS
shift in Hz
downfield
20
chemical
=
shift
parts per
million
shift in Hz
= ppm
=
spectrometer frequency in MHz
O
F
H 4 6 H =( - )f &
A %9
42
Dr. USAMA El-AYAAN
21
Delta Scale
22
=>
Location of Signals
More electronegative
atoms deshield more and
give larger shift values.
Effect decreases with
distance.
Additional
electronegative atoms
cause increase in chemical
shift.
Dr. USAMA El-AYAAN
23
24
Typical Values
25
13C
12C
is not NMRNMR-active
however.
13C
NMR h
I= 0
0 - 220 26ppm
Typical 13C
13C
NMR h
NMR
27
Fax
Feq
Feq
Feq
Feq
Feq doublet
Fax quintet
28
Brisdon, p. 34
31P
NMR Spectroscopy
Compound
PMe3
PEt3
PBu3
PPh3
[PEt4]+
[PF6]-
(ppm)
-62
-20
-33
-7
+40
-145
Compound
Me3PO
Et3PO
Bu3PO
Ph3PO
P(OMe)3
P(OEt)3
(ppm)
+36
+48
+43
+29
+140
+138
29
31P
R3P
R3P
Cl
Cl
R3P
M
Cl
R3P
PR3
fac-[M(PR3)3Cl3]
All three PR3 ligands are in
the same chemical
environment, so the 31P
NMR spectrum would have
only one resonance
Cl
M
Cl
PR3
mer-[M(PR3)3Cl3]
There are two PR3 ligands are in
the same chemical environment
(trans to each other) and the other
in a different environment, so the
31P NMR spectrum would have two
resonances, with a 2:1 relative
integration
30
31P
R3P
R3P
Cl
Cl
R3P
M
Cl
R3P
PR3
fac-[M(PR3)3Cl3]
All three PR3 ligands are in
the same chemical
environment, so the 31P
NMR spectrum would have
only one resonance
Cl
M
Cl
PR3
mer-[M(PR3)3Cl3]
There are two PR3 ligands are in
the same chemical environment
(trans to each other) and the other
in a different environment, so the
31P NMR spectrum would have two
resonances, with a 2:1 relative
integration
31
19F
F
F
F
P
F
F
32
Coupling
Consider one H split by one F
33
Brisdon, p. 36
Coupling
I = 1/2
34
Brisdon, p. 37
Coupling
I = 1/2
35
Brisdon, p. 38
Si(OCH2CH3)2
J = ((4
4.377377- 4.342
342))ppm x 200 MHz = 7 Hz
36
19F
doublet
37
Brisdon, p. 38
31P
NMR
spectrum
1J (axial)
PF
A doublet of
triplets
1J (equitorial)
PF
19F
NMR
spectrum
31P
39
40